ArticleJournal of structural biology: X2020
Improvements on marker-free images alignment for electron tomography.
Article in Journal of structural biology: X, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Fast automatic multiscale electron tomography for sensitive materials under environmental conditions.Communications engineering · 2025Article
- Article
- Automatic Differentiation for Inverse Problems in X-ray Imaging and Microscopy.Life (Basel, Switzerland) · 2023Article
- Automatic synchrotron tomographic alignment schemes based on genetic algorithms and human-in-the-loop software.Journal of synchrotron radiation · 2023Article
- On bias, variance, overfitting, gold standard and consensus in single-particle analysis by cryo-electron microscopy.Acta crystallographica. Section D, Structural biology · 2022Article
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Authors and funding
7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Electron tomography is a technique to obtain three-dimensional structural information of samples. However, the technique is limited by shifts occurring during acquisition that need to be corrected before the reconstruction process. In 2009, we proposed an approach for post-acquisition alignment of tilt series images. This approach was marker-free, based on patch tracking and integrated in free software. Here, we present improvements to the method to make it more reliable, stable and accurate. In addition, we modified the image formation model underlying the alignment procedure to include different deformations occurring during acquisition. We propose a new way to correct these computed deformations to obtain reconstructions with reduced artifacts. The new approach has demonstrated to improve the quality of the final 3D reconstruction, giving access to better defined structures for different transmission electron tomography methods: resin embedded STEM-tomography and cryo-TEM tomography. The method is freely available in TomoJ software.
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Registered trials
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