ArticleLight, science & applications2020
Converting lateral scanning into axial focusing to speed up three-dimensional microscopy.
Article in Light, science & applications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Two-photon light-sheet microscopy with isotropic near-diffraction-limited resolution in three dimensions.iScience · 2026Article
- Rapid axially scanned and de-scanned line-scan confocal microscopy with a tunable acoustic gradient index of refraction lens for high-speed volumetricNeurophotonics · 2025Article
- Intraoperative Fast Adaptive Focus Tracking Robotic OCT Enables Real-Time Tumor Grading and Large-Area Microvascular Imaging in Human Spinal Cord Surgery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Article
- Highly sensitive volumetric single-molecule imaging.Nanophotonics (Berlin, Germany) · 2024Article
- Remote refocusing for multi-scale imaging.Journal of biomedical optics · 2024Article
- Axial de-scanning using remote focusing in the detection arm of light-sheet microscopy.Nature communications · 2024Article
- Axial de-scanning using remote focusing in the detection arm of light-sheet microscopy.Research square · 2023Article
- Recent advances in oblique plane microscopy.Nanophotonics (Berlin, Germany) · 2023Review
- A Compact Two-Dimensional Varifocal Scanning Imaging Device Actuated by Artificial Muscle Material.Biomimetics (Basel, Switzerland) · 2023Article
- Efficient 3D light-sheet imaging of very large-scale optically cleared human brain and prostate tissue samples.Communications biology · 2023Article
- Varifocal MEMS mirrors for high-speed axial focus scanning: a review.Microsystems & nanoengineering · 2023Review
- Axial scanning of dual focus to improve light sheet microscopy.Biomedical optics express · 2022Article
- Isotropic imaging across spatial scales with axially swept light-sheet microscopy.Nature protocols · 2022Review
- Fast volumetric imaging with line-scan confocal microscopy by electrically tunable lens at resonant frequency.Optics express · 2022Article
- Neurophotonic tools for microscopic measurements and manipulation: status report.Neurophotonics · 2022Article
- Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adultFrontiers in neuroscience · 2022Article
- Advanced high resolution three-dimensional imaging to visualize the cerebral neurovascular network in stroke.International journal of biological sciences · 2022Review
- Electro-responsive actuators based on graphene.Innovation (Cambridge (Mass.)) · 2021Review
- CompassLSM: axially swept light-sheet microscopy made simple.Biomedical optics express · 2021Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
In optical microscopy, the slow axial scanning rate of the objective or the sample has traditionally limited the speed of volumetric imaging. Recently, by conjugating either a movable mirror to the image plane in a remote-focusing geometry or an electrically tuneable lens (ETL) to the back focal plane, rapid axial scanning has been achieved. However, mechanical actuation of a mirror limits the axial scanning rate (usually only 10-100 Hz for piezoelectric or voice coil-based actuators), while ETLs introduce spherical and higher-order aberrations that prevent high-resolution imaging. In an effort to overcome these limitations, we introduce a novel optical design that transforms a lateral-scan motion into a spherical aberration-free axial scan that can be used for high-resolution imaging. Using a galvanometric mirror, we scan a laser beam laterally in a remote-focusing arm, which is then back-reflected from different heights of a mirror in the image space. We characterize the optical performance of this remote-focusing technique and use it to accelerate axially swept light-sheet microscopy by an order of magnitude, allowing the quantification of rapid vesicular dynamics in three dimensions. We also demonstrate resonant remote focusing at 12 kHz with a two-photon raster-scanning microscope, which allows rapid imaging of brain tissues and zebrafish cardiac dynamics with diffraction-limited resolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.