Evidence map›Paper›PMID 33024248›Full record

ArticleThe pharmacogenomics journal2021

Characterization of four BCHE mutations associated with prolonged effect of suxamethonium.

Xavier Brazzolotto, Sébastien Courcelle, Christophe Sauvanet, Virginia Guillon, Alexandre Igert, Joseph Kononchik, Florian Nachon, Franck Ceppa, Hervé Delacour

Abstract read
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In one paragraph

Article in The pharmacogenomics journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Xavier BrazzolottoInstitut de Recherche Biomédicale des Armées, Division Défense NRBC, Département de Toxicologie et Risques Chimiques, 1 place Général Valérie André, B.P. 73, 91223, Brétigny sur Orge Cedex, France.ORCID http://orcid.org/0000-0001-9219-1032
Sébastien CourcelleHôpital d'Instruction des Armées Bégin, Fédération des Laboratoires, 69 avenue de Paris, 94163, Saint-Mandé Cedex, France.
Christophe SauvanetHôpital d'Instruction des Armées Bégin, Fédération des Laboratoires, 69 avenue de Paris, 94163, Saint-Mandé Cedex, France.
Virginia GuillonInstitut de Recherche Biomédicale des Armées, Division Défense NRBC, Département de Toxicologie et Risques Chimiques, 1 place Général Valérie André, B.P. 73, 91223, Brétigny sur Orge Cedex, France.
Alexandre IgertInstitut de Recherche Biomédicale des Armées, Division Défense NRBC, Département de Toxicologie et Risques Chimiques, 1 place Général Valérie André, B.P. 73, 91223, Brétigny sur Orge Cedex, France.
Joseph KononchikInstitut de Recherche Biomédicale des Armées, Division Défense NRBC, Département de Toxicologie et Risques Chimiques, 1 place Général Valérie André, B.P. 73, 91223, Brétigny sur Orge Cedex, France.
Florian NachonInstitut de Recherche Biomédicale des Armées, Division Défense NRBC, Département de Toxicologie et Risques Chimiques, 1 place Général Valérie André, B.P. 73, 91223, Brétigny sur Orge Cedex, France.ORCID http://orcid.org/0000-0003-0293-2429
Franck CeppaHôpital d'Instruction des Armées Bégin, Fédération des Laboratoires, 69 avenue de Paris, 94163, Saint-Mandé Cedex, France.ORCID http://orcid.org/0000-0002-1417-4111
Hervé DelacourHôpital d'Instruction des Armées Bégin, Fédération des Laboratoires, 69 avenue de Paris, 94163, Saint-Mandé Cedex, France. herve.delacour@intradef.gouv.fr.ORCID http://orcid.org/0000-0002-1150-0209
HIA du Val-de-Grâce à Paris · FRHôpital d'Instruction des Armées Bégin · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Butyrylcholinesterase (BChE) deficiency is characterized by prolonged apnea after the use of muscle relaxants (suxamethonium or mivacurium) in patients who have mutations in the BCHE gene. Here, we report the characterization of four BCHE mutations associated with prolonged effect of suxamethonium (amino acid numbering based on the matured enzyme): p.20delValPheGlyGlyThrValThr, p.Leu88His, p.Ile140del and p.Arg386Cys. Expression of recombinant BCHE mutants, kinetic analysis and molecular dynamics were undertaken to understand how these mutations induce BChE deficiency. Three of the mutations studied (p.20delValPheGlyGlyThrValThr, p.Ile140del and p.Arg386Cys) lead to a "silent" BChE phenotype. Recombinant BCHE expression studies for these mutants revealed BChE activity levels comparable to untransfected cells. Only the last one (hBChE-L88H) presented BChE activity in the transfected cell culture medium. This BChE mutant (p.Leu88His) is associated with a lower k

Indexed as

AdultAged, 80 and overButyrylcholinesteraseFemaleHumansKineticsMiddle AgedMivacuriumMutationPhenotypeSuccinylcholineBCHE protein, humanButyrylcholinesteraseMivacuriumSuccinylcholine

Identifiers

PMID33024248
OpenAlexW3091884540

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.