ArticleScientific reports2020
Molecular changes associated with spontaneous phenotypic variation of Paenibacillus polymyxa, a commonly used biocontrol agent, and temperature-dependent control of variation.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 14 citations in OpenAlex.
- Fusaricidins producing Paenibacillus: a potential biocontrol agent against plant pathogens.Archives of microbiology · 2026Review
- Characterization and Biocontrol Efficacy of Bacillus velezensis GYUN-1190 against Apple Bitter Rot.The plant pathology journal · 2024Article
- Abh, AbrB3, and Spo0A play distinct regulatory roles during polymyxin synthesis inMicrobiology spectrum · 2024Article
- Genomic and phenotypic analyses revealFrontiers in microbiology · 2024Article
- Influence of phenotypic variation ofFrontiers in microbiology · 2024Article
- Complete genome sequence ofMicrobiology resource announcements · 2023Article
- Schizotrophic Sclerotinia sclerotiorum-Mediated Root and Rhizosphere Microbiome Alterations Activate Growth and Disease Resistance in Wheat.Microbiology spectrum · 2023Article
- Isolation and Identification Antagonistic BacteriumFrontiers in plant science · 2023Article
- Occurrence of phenotypic variation inBiotechnology reports (Amsterdam, Netherlands) · 2022Article
- Ecological Dynamics and Microbial Treatments against Oomycete Plant Pathogens.Plants (Basel, Switzerland) · 2021Review
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There has been a growing interest in deploying plant growth-promoting rhizobacteria (PGPR) as a biological control agent (BCA) to reduce the use of agrochemicals. Spontaneous phenotypic variation of PGPR, which causes the loss of traits crucial for biocontrol, presents a large obstacle in producing commercial biocontrol products. Here, we report molecular changes associated with phenotypic variation in Paenibacillus polymyxa, a PGPR widely used for biocontrol worldwide, and a simple cultural change that can prevent the variation. Compared to B-type (non-variant) cells of P. polymyxa strain E681, its phenotypic variant, termed as F-type, fails to form spores, does not confer plant growth-promoting effect, and displays altered colony and cell morphology, motility, antagonism against other microbes, and biofilm formation. This variation was observed in all tested strains of P. polymyxa, but the frequency varied among them. RNA-seq analysis revealed differential regulation of many genes involved in sporulation, flagella synthesis, carbohydrate metabolism, and antimicrobial production in F-type cells, consistent with their pleiotropic phenotypic changes. F-type cells's sporulation was arrested at stage 0, and the key sporulation gene spo0A was upregulated only in B-type cells. The phenotypic variation could be prevented by altering the temperature for growth. When E681 was cultured at 20 °C or lower, it exhibited no variation for 7 days and still reached ~ 10
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