ArticlemSystems2020
End-to-End Protocol for the Detection of SARS-CoV-2 from Built Environments.
Article in mSystems, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Evaluation of rRNA depletion methods for capturing the RNA virome from environmental surfaces.BMC research notes · 2023Article
- Predicting COVID-19 cases using SARS-CoV-2 RNA in air, surface swab and wastewater samples.The Science of the total environment · 2023Article
- Transmission of SARS-CoV-2 in the workplace: Key findings from a rapid review of the literature.Aerosol science and technology : the journal of the American Association for Aerosol Research · 2023Article
- The effect of COVID-19 on emergencies and pain among orthodontic patients attending a teaching hospital.Journal of medicine and life · 2022Article
- Capacitive biosensor based on vertically paired electrodes for the detection of SARS-CoV-2.Biosensors & bioelectronics · 2022Article
- COVID-19 Prediction using Genomic Footprint of SARS-CoV-2 in Air, Surface Swab and Wastewater Samples.medRxiv : the preprint server for health sciences · 2022Article
- A Rapid RT-LAMP Assay for SARS-CoV-2 with Colorimetric Detection Assisted by a Mobile Application.Diagnostics (Basel, Switzerland) · 2022Article
- One-Step Homogeneous Immunoassay for the Detection of Influenza Virus Using Switching Peptide and Graphene Quencher.Biochip journal · 2022Article
- Analysis of SARS-CoV-2 RNA Persistence across Indoor Surface Materials Reveals Best Practices for Environmental Monitoring Programs.mSystems · 2021Article
- Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications.Journal of biomolecular techniques : JBT · 2021Review
- Effectiveness of SARS-CoV-2 Decontamination and Containment in a COVID-19 ICU.International journal of environmental research and public health · 2021Article
- Occurrence of SARS-CoV-2 in Indoor Environments With Increased Circulation and Gathering of People.Frontiers in public health · 2021Article
- SARS-CoV-2 detection and genomic sequencing from hospital surface samples collected at UC Davis.PloS one · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors at 5 institutions in 2 countries.
Funding
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes coronavirus disease 2019, is a respiratory virus primarily transmitted person to person through inhalation of droplets or aerosols, laden with viral particles. However, as recent studies have shown, virions can remain infectious for up to 72 h on surfaces, which can lead to transmission through contact. Thus, a comprehensive study was conducted to determine the efficiency of protocols to recover SARS-CoV-2 from surfaces in built environments. This end-to-end (E2E) study showed that the effective combination for monitoring SARS-CoV-2 on surfaces includes using an Isohelix swab collection tool, DNA/RNA Shield as a preservative, an automated system for RNA extraction, and reverse transcriptase quantitative PCR (RT-qPCR) as the detection assay. Using this E2E approach, this study showed that, in some cases, noninfectious viral fragments of SARS-CoV-2 persisted on surfaces for as long as 8 days even after bleach treatment. Additionally, debris associated with specific built environment surfaces appeared to inhibit and negatively impact the recovery of RNA; Amerstat demonstrated the highest inhibition (>90%) when challenged with an inactivated viral control. Overall, it was determined that this E2E protocol required a minimum of 1,000 viral particles per 25 cm
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.