Evidence map›Paper›PMID 33023470›Full record

ArticleBMC developmental biology2020

Monocyte Chemoattractant Protein-1 stimulates the differentiation of rat stem and progenitor Leydig cells during regeneration.

Xiangcheng Zhan, Jingwei Zhang, Saiyang Li, Xiaolu Zhang, Linchao Li, Tiantian Song, Qunlong Liu, Jun Lu, Yunfei Xu, Ren-Shan Ge

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC developmental biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Xiangcheng ZhanDepartment of Urology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China.
Jingwei ZhangDepartment of Urology, Yijishan Hospital, Wannan Medical College, Wuhu, 241000, Anhui, China.
Saiyang LiDepartment of Urology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China.
Xiaolu ZhangDepartment of Urology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China.
Linchao LiDepartment of Anesthesiology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China.
Tiantian SongDepartment of Anesthesiology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China.
Qunlong LiuDepartment of Urology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China.
Jun LuDepartment of Urology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China.
Yunfei XuDepartment of Urology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China. xuyunfeibb@sina.com.
Ren-Shan GeDepartment of Anesthesiology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China. r_ge@yahoo.com.ORCID 0000-0002-3448-9823
Tongji University · CNWenzhou Medical University · CNWannan Medical College · CN

Funding

National Natural Science Foundation of China 81671446National Natural Science Foundation of China 81730042Wenzhou Municipal Science and Technology Bureau ZS2017009
6 · The paper itself

Abstract

backgroundMonocyte chemoattractant protein-1(MCP-1) is a chemokine secreted by Leydig cells and peritubular myoid cells in the rat testis. Its role in regulating the development of Leydig cells via autocrine and paracrine is still unclear. The objective of the current study was to investigate the effects of MCP-1 on Leydig cell regeneration from stem cells in vivo and on Leydig cell development in vitro.

resultsIntratesticular injection of MCP-1(10 ng/testis) into Leydig cell-depleted rat testis from post-EDS day 14 to 28 significantly increased serum testosterone and luteinizing hormone levels, up-regulated the expression of Leydig cell proteins, LHCGR, SCARB1, CYP11A1, HSD3B1, CYP17A1, and HSD17B3 without affecting progenitor Leydig cell proliferation, as well as increased ERK1/2 phosphorylation. MCP-1 (100 ng/ml) significantly increased medium testosterone levels and up-regulated LHCGR, CYP11A1, and HSD3B1 expression without affecting EdU incorporation into stem cells after in vitro culture for 7 days. RS102895, a CCR2 inhibitor, reversed MCP-1-mediated increase of testosterone level after culture in combination with MCP-1.

conclusionMCP-1 stimulates the differentiation of stem and progenitor Leydig cells without affecting their proliferation.

Indexed as

AnimalsCell DifferentiationChemokine CCL2Gene ExpressionLeydig CellsLuteinizing HormoneMaleRatsRegenerationStem CellsTestisTestosteroneChemokine CCL2Luteinizing HormoneTestosteroneDifferentiationMonocyte chemoattractant protein-1ProliferationStem Leydig cellsTestosterone

Identifiers

PMID33023470
PMCPMC7541273
OpenAlexW3092310314

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.