Evidence map›Paper›PMID 33022978›Full record

ArticlePlants (Basel, Switzerland)2020

Live Imaging of Phosphate Levels in Arabidopsis Root Cells Expressing a FRET-Based Phosphate Sensor.

Ana G L Assunção, Sisse K Gjetting, Michael Hansen, Anja T Fuglsang, Alexander Schulz

Abstract read
In one paragraph

Article in Plants (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ana G L AssunçãoDepartment of Plant and Environmental Sciences, Copenhagen Plant Science Center, University of Copenhagen, 1871 Frederiksberg C, Denmark.ORCID 0000-0002-5562-6807
Sisse K GjettingDepartment of Plant and Environmental Sciences, Copenhagen Plant Science Center, University of Copenhagen, 1871 Frederiksberg C, Denmark.ORCID 0000-0002-7173-3880
Michael HansenDepartment of Plant and Environmental Sciences, Copenhagen Plant Science Center, University of Copenhagen, 1871 Frederiksberg C, Denmark.
Anja T FuglsangDepartment of Plant and Environmental Sciences, Copenhagen Plant Science Center, University of Copenhagen, 1871 Frederiksberg C, Denmark.ORCID 0000-0003-1153-8394
Alexander SchulzDepartment of Plant and Environmental Sciences, Copenhagen Plant Science Center, University of Copenhagen, 1871 Frederiksberg C, Denmark.

Funding

Danmarks Frie Forskningsfond 274-07-0172Danmarks Frie Forskningsfond 9041-00182B
6 · The paper itself

Abstract

Phosphorous (P) is an essential macronutrient in all organisms serving various fundamental biological processes, and is one of the least available plant nutrients in the soil. The application of inorganic phosphate (Pi) fertilizers is frequent, but it has a high environmental and financial cost. Breeding crops for improved Pi use-efficiency is a promising plant-based solution to pursue a reduction of fertilizer dependency. Availability of tools for monitoring changes of plant cellular Pi concentration in real-time can contribute to advancing knowledge on the molecular basis of Pi transport and homeostasis in plants. Genetically encoded fluorescent sensors have provided new insight on cellular processes. Here, we show that two Pi Fluorescence Resonance Energy Transfer (FRET)-based sensors from the FLIPPi family, the low-affinity FLIPPi-30m and the high-affinity FLIPPi-4µ, can be expressed and analyzed in

Indexed as

Arabidopsis thalianaFLIPPiFRET sensorphosphatePi homeostasisplant nutrition

Identifiers

PMID33022978
PMCPMC7600243

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.