ArticleThe EMBO journal2020
Glycolysis regulates Hedgehog signalling via the plasma membrane potential.
Article in The EMBO journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Wireless Bioelectronic Modulation of Membrane Potential in Glioblastoma Using Carbon Nanotube Porins.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Neuronal glycolytic reprogramming drives lethality via accelerated aging in abioRxiv : the preprint server for biology · 2025Article
- Hemocytes facilitate interclonal cooperation-induced tumor malignancy by hijacking the innate immune system in Drosophila.The EMBO journal · 2025Article
- The role of RNA methylation in tumor immunity and its potential in immunotherapy.Molecular cancer · 2024Review
- Historic obstacles and emerging opportunities in the field of developmental metabolism - lessons from Heidelberg.Development (Cambridge, England) · 2024Review
- Metabolomic analysis of Drosophila melanogaster larvae lacking pyruvate kinase.G3 (Bethesda, Md.) · 2023Article
- Metabolomic analysis ofbioRxiv : the preprint server for biology · 2023Article
- Hedgehog signaling can enhance glycolytic ATP production in the Drosophila wing disc.EMBO reports · 2022Article
- Targeting AKT and CK2 represents a novel therapeutic strategy for SMO constitutive activation-driven medulloblastoma.CNS neuroscience & therapeutics · 2022Article
- The role of m6A RNA methylation in cancer metabolism.Molecular cancer · 2022Review
- Cultivation and Live Imaging of Drosophila Imaginal Discs.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Optical Estimation of Absolute Membrane Potential Using One- and Two-Photon Fluorescence Lifetime Imaging Microscopy.Bioelectricity · 2021Article
- Membrane potential regulates Hedgehog signalling in the Drosophila wing imaginal disc.EMBO reports · 2021Article
- A Note of Caution: Gramicidin Affects Signaling Pathways Independently of Its Effects on Plasma Membrane Conductance.BioMed research international · 2021Article
- Glycolysis regulates Hedgehog signalling via the plasma membrane potential.The EMBO journal · 2020Article
- Getting in shape: ATP pumps up the volume in Hh signalling.The EMBO journal · 2020Article
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
Changes in cell metabolism and plasma membrane potential have been linked to shifts between tissue growth and differentiation, and to developmental patterning. How such changes mediate these effects is poorly understood. Here, we use the developing wing of Drosophila to investigate the interplay between cell metabolism and a key developmental regulator-the Hedgehog (Hh) signalling pathway. We show that reducing glycolysis both lowers steady-state levels of ATP and stabilizes Smoothened (Smo), the 7-pass transmembrane protein that transduces the Hh signal. As a result, the transcription factor Cubitus interruptus accumulates in its full-length, transcription activating form. We show that glycolysis is required to maintain the plasma membrane potential and that plasma membrane depolarization blocks cellular uptake of N-acylethanolamides-lipoprotein-borne Hh pathway inhibitors required for Smo destabilization. Similarly, pharmacological inhibition of glycolysis in mammalian cells induces ciliary translocation of Smo-a key step in pathway activation-in the absence of Hh. Thus, changes in cell metabolism alter Hh signalling through their effects on plasma membrane potential.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.