ArticleAmerican journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons2021
Urine recirculation prolongs normothermic kidney perfusion via more optimal metabolic homeostasis-a proteomics study.
Article in American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Perfusate Composition and Duration ofTransplant international : official journal of the European Society for Organ Transplantation · 2022Pooled it
- Urine Recirculation During Normothermic Kidney Preservation Improves Energy Balance Involving the Urea and Citric Acid Cycles.Transplantation direct · 2026Article
- Urine Proteomics as a Source of Biological Information and Outcome Predictor in Living Kidney Transplantation.Molecular & cellular proteomics : MCP · 2026Article
- Optimizing Prolonged (6 h) Normothermic Machine Perfusion of Donor Kidneys (PROPER Study).Artificial organs · 2026Article
- Comparison of Normothermic and Subnormothermic Machine Perfusion of Porcine Kidneys Using a Novel Fully Synthetic Perfusion Solution: A Proof-of-Concept Study.Journal of clinical medicine · 2026Article
- Time as a Therapeutic Ally: The Promise of Long-Term Solid Organ and Tissue Perfusion.Transplant international : official journal of the European Society for Organ Transplantation · 2026Review
- Simultaneous Ex Situ Normothermic Perfusion of Paired Kidneys in Pigs.Artificial organs · 2025Article
- Deceased donor kidney function and branched chain amino acid metabolism during ex vivo normothermic perfusion.Kidney international · 2024Article
- A cell-free nutrient-supplemented perfusate allows four-day ex vivo metabolic preservation of human kidneys.Nature communications · 2024Article
- Proteomics reveals specific biological changes induced by the normothermic machine perfusion of donor kidneys with a significant up-regulation of Latexin.Scientific reports · 2023Article
- Taurine as Antioxidant in a Novel Cell- and Oxygen Carrier-Free Perfusate for Normothermic Machine Perfusion of Porcine Kidneys.Antioxidants (Basel, Switzerland) · 2023Article
- Biomarker-Development Proteomics in Kidney Transplantation: An Updated Review.International journal of molecular sciences · 2023Review
- The impact of oxygen supply and erythrocytes during normothermic kidney perfusion.Scientific reports · 2023Article
- Article
- Application of Ex Vivo Normothermic Machine Perfusion in Deceased Donors With Acute Kidney Injury With Successful Renal Transplantation: A Preliminary Experience.Transplantation direct · 2022Article
- Forty-eight hours of normothermic kidney preservation applying urine recirculation.Artificial organs · 2022Article
- A Review of Current and Emerging Trends in Donor Graft-Quality Assessment Techniques.Journal of clinical medicine · 2022Review
- Ex Vivo Mesenchymal Stem Cell Therapy to Regenerate Machine Perfused Organs.International journal of molecular sciences · 2021Review
- Hemodynamics and Metabolic Parameters in Normothermic Kidney Preservation Are Linked With Donor Factors, Perfusate Cells, and Cytokines.Frontiers in medicine · 2021Article
- Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
We describe a proteomics analysis to determine the molecular differences between normothermically perfused (normothermic machine perfusion, NMP) human kidneys with urine recirculation (URC) and urine replacement (UR). Proteins were extracted from 16 kidney biopsies with URC (n = 8 donors after brain death [DBD], n = 8 donors after circulatory death [DCD]) and three with UR (n = 2 DBD, n = 1 DCD), followed by quantitative analysis by mass spectrometry. Damage-associated molecular patterns (DAMPs) were decreased in kidney tissue after 6 hours NMP with URC, suggesting reduced inflammation. Vasoconstriction was also attenuated in kidneys with URC as angiotensinogen levels were reduced. Strikingly, kidneys became metabolically active during NMP, which could be enhanced and prolonged by URC. For instance, mitochondrial succinate dehydrogenase enzyme levels as well as carbonic anhydrase were enhanced with URC, contributing to pH stabilization. Levels of cytosolic and the mitochondrial phosphoenolpyruvate carboxykinase were elevated after 24 hours of NMP, more prevalent in DCD than DBD tissue. Key enzymes involved in glucose metabolism were also increased after 12 and 24 hours of NMP with URC, including mitochondrial malate dehydrogenase and glutamic-oxaloacetic transaminase, predominantly in DCD tissue. We conclude that NMP with URC permits prolonged preservation and revitalizes metabolism to possibly better cope with ischemia reperfusion injury in discarded kidneys.
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