ArticleNature communications2020
Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Dynamic regulation of endogenous transcription factor hubs at single-molecule resolution.bioRxiv : the preprint server for biology · 2026Article
- Enhanced spatial clustering of single-molecule localizations with graph neural networks.Nature communications · 2025Article
- Facile construction of dual-response super-resolution probes for tracking organelles dynamics.Exploration (Beijing, China) · 2024Article
- CD3 aptamers promote expansion and persistence of tumor-reactive T cells for adoptive T cell therapy in cancer.Molecular therapy. Nucleic acids · 2024Article
- Quantum-enhanced diamond molecular tension microscopy for quantifying cellular forces.Science advances · 2024Article
- Monomeric agonist peptide/MHCII complexes activate T-cells in an autonomous fashion.EMBO reports · 2023Article
- Correction of multiple-blinking artifacts in photoactivated localization microscopy.Nature methods · 2022Article
- Understanding immune signaling using advanced imaging techniques.Biochemical Society transactions · 2022Review
- Don't Be Fooled by Randomness: ValidFrontiers in bioinformatics · 2022Review
- Strategies for the Site-Specific Decoration of DNA Origami Nanostructures with Functionally Intact Proteins.ACS nano · 2021Article
- A high-throughput DNA FISH protocol to visualize genome regions in human cells.STAR protocols · 2021Article
- MUW researcher of the month.Wiener klinische Wochenschrift · 2021Article
- Membrane Nanoscopic Organization of D2L Dopamine Receptor Probed by Quantum Dot Tracking.Membranes · 2021Article
- DNA origami demonstrate the unique stimulatory power of single pMHCs as T cell antigens.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Role of Protein and Lipid Clustering in Lymphocyte Activation.Frontiers in immunology · 2021Review
- Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy.Nature communications · 2020Article
- The cytoskeletal regulator HEM1 governs B cell development and prevents autoimmunity.Science immunology · 2020Article
- Direct Regulation of the T Cell Antigen Receptor's Activity by Cholesterol.Frontiers in cell and developmental biology · 2020Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Determining nanoscale protein distribution via Photoactivated Localization Microscopy (PALM) mandates precise knowledge of the applied fluorophore's blinking properties to counteract overcounting artifacts that distort the resulting biomolecular distributions. Here, we present a readily applicable methodology to determine, optimize and quantitatively account for the blinking behavior of any PALM-compatible fluorophore. Using a custom-designed platform, we reveal complex blinking of two photoswitchable fluorescence proteins (PS-CFP2 and mEOS3.2) and two photoactivatable organic fluorophores (PA Janelia Fluor 549 and Abberior CAGE 635) with blinking cycles on time scales of several seconds. Incorporating such detailed information in our simulation-based analysis package allows for robust evaluation of molecular clustering based on individually recorded single molecule localization maps.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.