ArticleInternational journal of molecular sciences2020
Identification of the Lineage Markers and Inhibition of
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- IGF-1 promotes trophectoderm cell proliferation of porcine embryos by activating the Wnt/β-catenin pathway.Cell communication and signaling : CCS · 2025Article
- NANOG expression in parthenogenetic porcine blastocysts is required for intact lineage specification and pluripotency.Animal bioscience · 2023Article
- The number of primitive endoderm cells in the inner cell mass is regulated by platelet-derived growth factor signaling in porcine preimplantation embryos.Animal bioscience · 2023Article
- Comparison data of transcriptomes from blastocyst seeding samples and cultured cell lines from pigs.Data in brief · 2023Article
- Changes in OCT4 expression play a crucial role in the lineage specification and proliferation of preimplantation porcine blastocysts.Cell proliferation · 2022Article
- The Development, Differentiation, and Toxicity in Reproduction.International journal of molecular sciences · 2022Article
- Characterization of multitype colonies originating from porcine blastocysts producedFrontiers in cell and developmental biology · 2022Article
- SOX2 plays a crucial role in cell proliferation and lineage segregation during porcine pre-implantation embryo development.Cell proliferation · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Specification of embryonic lineages is an important question in the field of early development. Numerous studies analyzed the expression patterns of the candidate transcripts and proteins in humans and mice and clearly determined the markers of each lineage. To overcome the limitations of human and mouse embryos, the expression of the marker transcripts in each cell has been investigated using in vivo embryos in pigs. In vitro produced embryos are more accessible, can be rapidly processed with low cost. Therefore, we analyzed the characteristics of lineage markers and the effects of the DAB2 gene (trophectoderm marker) in in vitro fertilized porcine embryos. We investigated the expression levels of the marker genes during embryonic stages and distribution of the marker proteins was assayed in day 7 blastocysts. Then, the shRNA vectors were injected into the fertilized embryos and the differences in the marker transcripts were analyzed. Marker transcripts showed diverse patterns of expression, and each embryonic lineage could be identified with localization of marker proteins. In DAB2-shRNA vectors injected embryos, HNF4A and PDGFRA were upregulated. DAB2 protein level was lower in shRNA-injected embryos without significant differences. Our results will contribute to understanding of the mechanisms of embryonic lineage specification in pigs.
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Registered trials
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