ArticleACS synthetic biology2020
Construction of Fully Segregated Genomic Libraries in Polyploid Organisms Such as
Article in ACS synthetic biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed, 7 citations in OpenAlex.
- Determinants ofGenes · 2024Article
- The Molecular Toolset and Techniques Required to Build Cyanobacterial Cell Factories.Advances in biochemical engineering/biotechnology · 2023Article
- Synthetic counter-selection markers and their application in genetic modification of Synechococcus elongatus UTEX2973.Applied microbiology and biotechnology · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
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Abstract
Several microbes are polyploid, meaning they contain several copies of their chromosome. Cyanobacteria, while holding great potential as photosynthetic cell factories of various products, are found among them. In these clades the diversity of genetic elements that serve within the basic molecular toolbox is often limiting. To assist mining for the latter, we present here a method for the generation of fully segregated genomic libraries, specifically designed for polyploids. We provide proof-of-principle for this method by generating a fully segregated genomic promoter library in the cyanobacterium
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