ArticleJournal of veterinary science2020
Development of a biosensor from aptamers for detection of the porcine reproductive and respiratory syndrome virus.
Article in Journal of veterinary science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Microfluidic-optical integrated portable platform with handheld pump for wash-free plasmonic detection of thrombin and SARS-CoV-2 spike protein.Microsystems & nanoengineering · 2026Article
- A countrywide survey of PRRS indicates widespread seroprevalence in India with age of the pig and zone as significant risk factors.Virusdisease · 2025Article
- Recent Advances in Biosensor Technologies for Meat Production Chain.Foods (Basel, Switzerland) · 2025Review
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Authors and funding
5 authors.
Funding
Abstract
backgroundRecently, the pork industry of Thailand faced an epidemic of highly virulent strains of porcine reproductive and respiratory syndrome virus (PRRSV), which spread throughout Southeast Asia, including the Lao People's Democratic Republic and Cambodia. Hence, the rapid and on-site screening of infected pigs on a farm is essential.
objectivesTo develop the new aptamer as a biosensor for detection PRRSV which are rapid and on-site screening of infected pig.
methodsNew aptamers against PRSSV were identified using the combined techniques of capillary electrophoresis, colorimetric assay by gold nanoparticles, and quartz crystal microbalance (QCM).
resultsThirty-six candidate aptamers of the PRRSV were identified from the systematic evolution of ligands by exponential enrichment (SELEX) by capillary electrophoresis. Only 8 out of 36 aptamers could bind to the PRSSV, as shown in a colorimetric assay. Of the 8 aptamers tested, only the 1F aptamer could bind specifically to the PRSSV when presented with the classical swine fever virus and a pseudo rabies virus. The QCM was used to confirm the specificity and sensitivity of the 1F aptamer with a detection limit of 1.87 × 10
conclusionsSELEX screening of the aptamer equipped with capillary electrophoresis potentially revealed promising candidates for detecting the PRRSV. The 1F aptamer exhibited the highest specificity and selectivity against the PRRSV. These findings suggest that 1F is a promising aptamer for further developing a novel PRRSV rapid detection kit.
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