Evidence map›Paper›PMID 33016018›Full record

ArticleJournal of veterinary science2020

Development of a novel reverse transcription PCR and its application to field sample testing for feline calicivirus prevalence in healthy stray cats in Korea.

Sung Jae Kim, Yong Ho Park, Kun Taek Park

Open access · goldAbstract read
In one paragraph

Article in Journal of veterinary science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
0.2field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 16 citations in OpenAlex.

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  4. Epidemiology and co-infection of URTD pathogens in domestic cats during COVID-19.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Sung Jae KimDepartment of Veterinary Microbiology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.ORCID https://orcid.org/0000-0002-8813-012X
Yong Ho ParkDepartment of Veterinary Microbiology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.ORCID https://orcid.org/0000-0002-4168-6884
Kun Taek ParkDepartment of Biotechnology, Inje University, Gimhae 50834, Korea. ktpark@inje.ac.kr.ORCID https://orcid.org/0000-0001-6177-0373
Seoul National University · KRInje University · KR

Funding

Inje University
6 · The paper itself

Abstract

backgroundFeline calicivirus (FCV) is a major and highly infectious pathogen in cats worldwide. However, there have been limited studies about the status of FCV infections in Korea.

objectivesTo investigate the current status of FCV infections in stray cats in Korea.

methodsA novel reverse transcription polymerase chain reaction (RT-PCR) assay was developed based on the conserved nucleotide sequences of reported FCV strains. Field swab samples were collected from 122 cats (2 hospital admitted cats and 120 stray cats) in 2016 and 2017. All the samples were tested by virus isolation and 2 different RT-PCRs, including the novel RT-PCR, for the detection of FCV.

resultsThe novel RT-PCR assay showed no cross-reactivity to the nucleic acids of the other feline pathogens tested, and the limit of detection was calculated as 10⁰ TCID

conclusionsThe current study is the first report of the detection and prevalence of FCV in stray cats in Korea. The novel RT-PCR assay developed in this study showed high sensitivity and specificity, which indicates a useful diagnostic assay to identify FCV infection in cats.

Indexed as

AnimalsCaliciviridae InfectionsCalicivirus, FelineCat DiseasesCatsPrevalenceRepublic of KoreaReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityFeline calicivirusprevalencereverse transcriptase PCR

Identifiers

PMID33016018
PMCPMC7533393
OpenAlexW3083531937

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.