Evidence map›Paper›PMID 33014576›Full record

ArticleBiomedical optics express2020

Planar Airy beam light-sheet for two-photon microscopy.

Neveen A Hosny, James A Seyforth, Gunnar Spickermann, Thomas J Mitchell, Pedro Almada, Robert Chesters, Scott J Mitchell, George Chennell, Anthony C Vernon, Kwangwook Cho and 3 more

Abstract read
In one paragraph

Article in Biomedical optics express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Space-time light-sheet microscopy.Nature communications · 2026
    Article
  3. Article
  4. Review
  5. Article
  6. Video-rate Raman-based metabolic imaging by Airy light-sheet illumination and photon-sparse detection.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  7. Article
  8. Article
  9. FastBiomedical optics express · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Neveen A HosnyM Squared Life, The Surrey Technology Centre, Guildford, Surrey, GU2 7YG, UK.ORCID https://orcid.org/0000-0003-2224-4164
James A SeyforthM Squared Life, The Surrey Technology Centre, Guildford, Surrey, GU2 7YG, UK.
Gunnar SpickermannM Squared Life, The Surrey Technology Centre, Guildford, Surrey, GU2 7YG, UK.ORCID https://orcid.org/0000-0003-4822-8381
Thomas J MitchellM Squared Life, The Surrey Technology Centre, Guildford, Surrey, GU2 7YG, UK.ORCID https://orcid.org/0000-0003-4534-8505
Pedro AlmadaM Squared Life, The Surrey Technology Centre, Guildford, Surrey, GU2 7YG, UK.
Robert ChestersBasic and Clinical Neuroscience Department, King's College London, London, SE5 9NU, UK.
Scott J MitchellBasic and Clinical Neuroscience Department, King's College London, London, SE5 9NU, UK.
George ChennellBasic and Clinical Neuroscience Department, King's College London, London, SE5 9NU, UK.
Anthony C VernonBasic and Clinical Neuroscience Department, King's College London, London, SE5 9NU, UK.
Kwangwook ChoBasic and Clinical Neuroscience Department, King's College London, London, SE5 9NU, UK.
Deepak P SrivastavaBasic and Clinical Neuroscience Department, King's College London, London, SE5 9NU, UK.ORCID https://orcid.org/0000-0001-6512-9065
Robert ForsterM Squared Life, The Surrey Technology Centre, Guildford, Surrey, GU2 7YG, UK.
Tom VettenburgSchool of Physics and Astronomy, University of Exeter, EX4 4QL, Exeter, UK.ORCID https://orcid.org/0000-0002-5508-1130

Funding

Medical Research Council MR/L021064/1Medical Research Council MR/N025377/1Medical Research Council MR/N026063/1Medical Research Council MR/S034900/1
6 · The paper itself

Abstract

We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-fold increase in field-of-view with single-photon excitation; however, the characteristic asymmetry of the light-sheet limits its potential for multi-photon excitation. Here we show how a planar light-sheet can be formed from the curved propagation-invariant Airy beam. The resulting symmetric light sheet excites two-photon fluorescence uniformly across an extended field-of-view without the need for deconvolution. We demonstrate the method for rapid two-photon imaging of large volumes of neuronal tissue.

Identifiers

PMID33014576
PMCPMC7510906

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.