Evidence map›Paper›PMID 33013853›Full record

ArticleFrontiers in immunology2020

Validation of a Combined Transcriptome and T Cell Receptor Alpha/Beta (TRA/TRB) Repertoire Assay at the Single Cell Level for Paucicellular Samples.

Nicolle H R Litjens, Anton W Langerak, Amy C J van der List, Mariska Klepper, Maaike de Bie, Zakia Azmani, Alexander T den Dekker, Rutger W W Brouwer, Michiel G H Betjes, Wilfred F J Van IJcken

Open access · goldAbstract read
In one paragraph

Article in Frontiers in immunology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Nicolle H R LitjensDepartment of Internal Medicine Section Nephrology and Transplantation, Erasmus MC, University Medical Center, Rotterdam, Netherlands.
Anton W LangerakLaboratory Medical Immunology, Department of Immunology, Erasmus MC, University Medical Center, Rotterdam, Netherlands.
Amy C J van der ListDepartment of Internal Medicine Section Nephrology and Transplantation, Erasmus MC, University Medical Center, Rotterdam, Netherlands.
Mariska KlepperDepartment of Internal Medicine Section Nephrology and Transplantation, Erasmus MC, University Medical Center, Rotterdam, Netherlands.
Maaike de BieLaboratory Medical Immunology, Department of Immunology, Erasmus MC, University Medical Center, Rotterdam, Netherlands.
Zakia AzmaniCenter for Biomics, Erasmus MC, University Medical Center, Rotterdam, Netherlands.
Alexander T den DekkerCenter for Biomics, Erasmus MC, University Medical Center, Rotterdam, Netherlands.
Rutger W W BrouwerCenter for Biomics, Erasmus MC, University Medical Center, Rotterdam, Netherlands.
Michiel G H BetjesDepartment of Internal Medicine Section Nephrology and Transplantation, Erasmus MC, University Medical Center, Rotterdam, Netherlands.
Wilfred F J Van IJckenCenter for Biomics, Erasmus MC, University Medical Center, Rotterdam, Netherlands.
Erasmus MC · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Transcriptomics can be combined with TRA and TRB clonotype analysis at the single cell level. The aim of this study was to validate this approach on the ICELL8 Single-Cell system and to evaluate its usefulness to analyse clinical paucicellular samples. For this purpose, we carefully selected T cell lines with defined TRA/TRB clonotypes as well as clinical samples enriched for CD3

Indexed as

Cell LineClone CellsComputational BiologyFlow CytometryHigh-Throughput Nucleotide SequencingHumansReceptors, Antigen, T-Cell, alpha-betaSingle-Cell AnalysisT-LymphocytesTranscriptomeReceptors, Antigen, T-Cell, alpha-betacDNAcombined transcriptome and T cell receptor repertoire assaylow cell numbersingle cell transcriptomicssingle T cell receptor repertoire analysis

Identifiers

PMID33013853
PMCPMC7500136
OpenAlexW3082281261

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.