Evidence map›Paper›PMID 33013811›Full record

ArticleFrontiers in microbiology2020

German-Wide Interlaboratory Study Compares Consistency, Accuracy and Reproducibility of Whole-Genome Short Read Sequencing.

Laura Uelze, Maria Borowiak, Markus Bönn, Erik Brinks, Carlus Deneke, Thomas Hankeln, Sylvia Kleta, Larissa Murr, Kerstin Stingl, Kathrin Szabo and 3 more

Abstract read
In one paragraph

Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Article
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  5. Article
  6. Genotyping of European Toxoplasma gondii strains by a new high-resolution next-generation sequencing-based method.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024
    Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. Review
  13. Taxonomic Evaluation of theMicroorganisms · 2021
    Article
  14. Article
  15. Article
  16. Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Laura UelzeDepartment of Biological Safety, German Federal Institute for Risk Assessment (BfR), Berlin, Germany.
Maria BorowiakDepartment of Biological Safety, German Federal Institute for Risk Assessment (BfR), Berlin, Germany.
Markus BönnLandesamt für Verbraucherschutz Sachsen-Anhalt (LAV), Halle (Saale), Germany.
Erik BrinksDepartment of Microbiology and Biotechnology, Max Rubner-Institut (MRI), Kiel, Germany.
Carlus DenekeDepartment of Biological Safety, German Federal Institute for Risk Assessment (BfR), Berlin, Germany.
Thomas HankelnInstitute of Organismic and Molecular Evolution, AG Molecular Genetics and Genome Analysis, Johannes Gutenberg Universität Mainz, Mainz, Germany.
Sylvia KletaDepartment of Biological Safety, German Federal Institute for Risk Assessment (BfR), Berlin, Germany.
Larissa MurrBavarian Health and Food Safety Authority (LGL), Oberschleißheim, Germany.
Kerstin StinglDepartment of Biological Safety, German Federal Institute for Risk Assessment (BfR), Berlin, Germany.
Kathrin SzaboDepartment 5, Federal Office of Consumer Protection and Food Safety (BVL), Berlin, Germany.
Simon H TauschDepartment of Biological Safety, German Federal Institute for Risk Assessment (BfR), Berlin, Germany.
Anne WöhlkeFood and Veterinary Institute, Lower Saxony State Office for Consumer Protection and Food Safety (LAVES), Braunschweig, Germany.
Burkhard MalornyDepartment of Biological Safety, German Federal Institute for Risk Assessment (BfR), Berlin, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We compared the consistency, accuracy and reproducibility of next-generation short read sequencing between ten laboratories involved in food safety (research institutes, state laboratories, universities and companies) from Germany and Austria. Participants were asked to sequence six DNA samples of three bacterial species (

Indexed as

food safetyilluminainterlaboratory studyion torrentwhole-genome sequencing

Identifiers

PMID33013811
PMCPMC7516015

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.