ArticleFrontiers in microbiology2020
Bioorthogonal Non-canonical Amino Acid Tagging Combined With Flow Cytometry for Determination of Activity in Aquatic Microorganisms.
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Seasonally and niche-differentiated diversity of active, dormant and dead microbes in coastal waters and surface sediments.Communications biology · 2025Article
- Using BONCAT to dissect the proteome ofmSphere · 2025Article
- Testing antimicrobial peptides inhibiting protein synthesis in livingFrontiers in microbiology · 2025Article
- At what cost? The impact of bacteriophage resistance on the growth kinetics and protein synthesis of Escherichia coli.Environmental microbiology reports · 2024Article
- Engineered Proteins and Materials Utilizing Residue-Specific Noncanonical Amino Acid Incorporation.Chemical reviews · 2024Review
- Unlocking the mechanism of action: a cost-effective flow cytometry approach for accelerating antimicrobial drug development.Microbiology spectrum · 2024Article
- Microbial Diversity and Activity of Biofilms from Geothermal Springs in Croatia.Microbial ecology · 2023Article
- Using click chemistry to study microbial ecology and evolution.ISME communications · 2023Review
- Opportunities in optical and electrical single-cell technologies to study microbial ecosystems.Frontiers in microbiology · 2023Review
- Insights into plastic biodegradation: community composition and functional capabilities of the superworm (Microbial genomics · 2022Article
- Flow Cytometric Analysis of Bacterial Protein Synthesis: Monitoring Vitality After Water Treatment.Frontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we have combined bioorthogonal non-canonical amino acid tagging (BONCAT) and flow cytometry (FCM) analysis, and we demonstrate the applicability of the method for marine prokaryotes. Enumeration of active marine bacteria was performed by combining the DNA stain SYBR Green with detection of protein production with BONCAT. After optimization of incubation condition and substrate concentration on monoculture of
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Registered trials
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