Evidence map›Paper›PMID 33009646›Full record

ArticleExperimental & applied acarology2020

Utilizing attached hard ticks as pointers to the risk of infection by Babesia and Theileria species in sika deer (Cervus nippon yesoensis), in Japan.

Elzahara Elbaz, Mohamed Abdallah Mohamed Moustafa, Kyunglee Lee, Alice Lau Ching Ching, Michito Shimozuru, Mariko Sashika, Ryo Nakao, Sabry Ahmed El-Khodery, Toshio Tsubota

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Experimental & applied acarology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. The Global Emergence of Human Babesiosis.Pathogens (Basel, Switzerland) · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 3 countries.

Elzahara ElbazLaboratory of Wildlife Biology and Medicine, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, North 18 West 9, Sapporo, Hokkaido, 060-0818, Japan.
Mohamed Abdallah Mohamed MoustafaLaboratory of Wildlife Biology and Medicine, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, North 18 West 9, Sapporo, Hokkaido, 060-0818, Japan.
Kyunglee LeeCetacean Research Institute, National Institute of Fisheries Science, Ulsan, Republic of Korea.
Alice Lau Ching ChingLaboratory of Wildlife Biology and Medicine, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, North 18 West 9, Sapporo, Hokkaido, 060-0818, Japan.
Michito ShimozuruLaboratory of Wildlife Biology and Medicine, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, North 18 West 9, Sapporo, Hokkaido, 060-0818, Japan.
Mariko SashikaLaboratory of Wildlife Biology and Medicine, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, North 18 West 9, Sapporo, Hokkaido, 060-0818, Japan.
Ryo NakaoLaboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Sabry Ahmed El-KhoderyDepartment of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Toshio TsubotaLaboratory of Wildlife Biology and Medicine, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, North 18 West 9, Sapporo, Hokkaido, 060-0818, Japan. tsubota@vetmed.hokudai.ac.jp.ORCID http://orcid.org/0000-0002-8314-1233
Hokkaido University · JPMansoura University · EGNational Institute of Fisheries Science · KRSouth Valley University · EG

Funding

Ministry of Education, Culture, Sports, Science and Technology F01
6 · The paper itself

Abstract

Ticks are hematophagous ectoparasites that have a significant impact on their animal hosts. Along with mosquitoes, they are the main arthropod vectors of disease agents in domestic animals, wildlife and humans. To investigate the occurrence and prevalence of piroplasmids in ticks, DNA was extracted from 519 hard ticks collected from 116 hunted Hokkaido sika deer (Cervus nippon yesoensis). The success of the DNA extraction was confirmed by touchdown PCR targeting the mitochondrial 16S rDNA gene of ticks. Touchdown PCR and reverse line blot (RLB) hybridization targeting the 18S rRNA gene were used to detect 14 piroplasm species. All hard ticks parasitizing Hokkaido sika deer were identified as belonging to the genera Ixodes and Haemaphysalis. In total 163 samples (31.4%) were positive for Babesia and Theileria spp. among tick species according to RLB hybridization. Tick DNA hybridized to the oligonucleotide probes of Theileria sp. Thrivae (27.0% of ticks; 140/519), Theileria capreoli (10.6%; 55/519), Babesia divergens-like (1.7%; 9/519), Babesia sp. (Bab-SD) (0.6%; 3/519), Babesia microti U.S. (0.4%; 2/519), and B. microti Hobetsu (0.4%; 2/519). The partial sequencing and phylogenetic analyses of the 18S rRNA gene confirmed the RLB hybridization results. Further investigations are needed to reveal the epidemiology and respective vectors of these pathogens.

Indexed as

BabesiaTheileriaAnimalsBabesiosisDeerDNA, ProtozoanIxodidaeJapanMosquito VectorsPhylogenyRNA, Ribosomal, 18STheileriasisDNA, ProtozoanRNA, Ribosomal, 18SBabesiaHard ticksReverse line blotSika deerTheileria

Identifiers

PMID33009646
OpenAlexW3091095168

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.