ArticleApplied biochemistry and biotechnology2021
Exploring the Milk-Clotting and Proteolytic Activities in Different Tissues of Vallesia glabra: a New Source of Plant Proteolytic Enzymes.
Article in Applied biochemistry and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 21 citations in OpenAlex.
- Proteases from an Amazonian Mushroom Species: A Mycotechnological Alternative for the Production of Milk Coagulant.Applied biochemistry and biotechnology · 2025Article
- The herbal pharmacopoeia of Ecuador: a national model for integrating traditional knowledge and biodiscovery.Frontiers in pharmacology · 2025Article
- Oilseed Extracts from Local Markets as Promising Coagulant Agents for Milk from Various Mammalian Species.Foods (Basel, Switzerland) · 2022Article
- Plant Milk-Clotting Enzymes for Cheesemaking.Foods (Basel, Switzerland) · 2022Review
- Constitutive expression ofHeliyon · 2021Article
- Hemoglobin Precipitation: An Index ofEvidence-based complementary and alternative medicine : eCAM · 2021Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
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Abstract
Proteolytic enzymes are widely distributed in nature, playing essential roles in important biological functions. Recently, the use of plant proteases at the industrial level has mainly increased in the food industry (e.g., cheesemaking, meat tenderizing, and protein hydrolysate production). Current technological and scientific advances in the detection and characterization of proteolytic enzymes have encouraged the search for new natural sources. Thus, this work aimed to explore the milk-clotting and proteolytic properties of different tissues of Vallesia glabra. Aqueous extracts from the leaves, fruits, and seeds of V. glabra presented different protein profiles, proteolytic activity, and milk-clotting activity. The milk-clotting activity increased with temperature (30-65 °C), but this activity was higher in leaf (0.20 MCU/mL) compared with that in fruit and seed extracts (0.12 and 0.11 MCU/mL, respectively) at 50 °C. Proteolytic activity in the extracts assayed at different pH (2.5-12.0) suggested the presence of different types of active proteases, with maximum activity at acidic conditions (4.0-4.5). Inhibitory studies indicated that major activity in V. glabra extracts is related to cysteine proteases; however, the presence of serine, aspartic, and metalloproteases was also evident. The hydrolytic profile of caseins indicated that V. glabra leaves could be used as a rennet substitute in cheesemaking, representing a new and promising source of proteolytic enzymes.
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