Evidence map›Paper›PMID 33009494›Full record

ArticleScientific reports2020

Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex.

Alina Minias, Lidia Żukowska, Jakub Lach, Tomasz Jagielski, Dominik Strapagiel, Su-Young Kim, Won-Jung Koh, Heather Adam, Ruth Bittner, Sara Truden and 2 more

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.8field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
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  3. Nontuberculous mycobacterial infection in Wisconsin children and adolescents.Journal of clinical tuberculosis and other mycobacterial diseases · 2024
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  7. Short SignatureMicrobiology spectrum · 2022
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  8. Phylogenomic analysis of the genusFrontiers in microbiology · 2022
    Article
  9. Article
  10. Microorganisms · 2021
    Article
  11. Review
  12. Cell surface (Amsterdam, Netherlands) · 2021
    Article
  13. Article
  14. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 7 institutions in 4 countries.

Alina MiniasInstitute of Medical Biology, Polish Academy of Sciences, ul. Lodowa 106, 93-232, Lodz, Poland. aminias@cbm.pan.pl.
Lidia ŻukowskaBioMedChem Doctoral School of the University of Lodz, The Institutes of the Polish Academy of Sciences, Lodz, Poland.
Jakub LachBiobank Lab, Department of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
Tomasz JagielskiDepartment of Medical Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
Dominik StrapagielBiobank Lab, Department of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
Su-Young KimDivision of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.
Won-Jung KohDivision of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.
Heather AdamDiagnostic Services, Shared Health, Winnipeg, MB, Canada.
Ruth BittnerDiagnostic Services, Shared Health, Winnipeg, MB, Canada.
Sara TrudenNational Reference Laboratory for Mycobacteria, University Clinic of Respiratory and Allergic Diseases, Golnik, Slovenia.
Manca Žolnir-DovčNational Reference Laboratory for Mycobacteria, University Clinic of Respiratory and Allergic Diseases, Golnik, Slovenia.
Jarosław DziadekInstitute of Medical Biology, Polish Academy of Sciences, ul. Lodowa 106, 93-232, Lodz, Poland.
University of Łódź · PLInstitute for Medical Biology · PLManitoba Health · CAUniversity Clinic of Pulmonary and Allergic Diseases Golnik · SISamsung Medical Center · KRSungkyunkwan University · KRUniversity of Warsaw · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mycobacterium abscessus complex (MABC) is a taxonomic group of rapidly growing, nontuberculous mycobacteria that are found as etiologic agents of various types of infections. They are considered as emerging human pathogens. MABC consists of 3 subspecies-M. abscessus subsp. bolletti, M. abscessus subsp. massiliense and M. abscessus subsp. abscessus. Here we present a novel method for subspecies differentiation of M. abscessus named Subspecies-Specific Sequence Detection (SSSD). This method is based on the presence of signature sequences present within the genomes of each subspecies of MABC. We tested this method against a virtual database of 1505 genome sequences of MABC. Further, we detected signature sequences of MABC in 45 microbiological samples through DNA hybridization. SSSD showed high levels of sensitivity and specificity for differentiation of subspecies of MABC, comparable to those obtained by rpoB sequence typing.

Indexed as

Bacterial ProteinsDNA, BacterialGenome, BacterialHumansMycobacterium abscessusMycobacterium Infections, NontuberculousSensitivity and SpecificitySequence Analysis, DNABacterial ProteinsDNA, Bacterial

Identifiers

PMID33009494
PMCPMC7532137
OpenAlexW3091340828

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.