ArticleAnimals : an open access journal from MDPI2020
In Vitro Culture of Chicken Circulating and Gonadal Primordial Germ Cells on a Somatic Feeder Layer of Avian Origin.
Article in Animals : an open access journal from MDPI, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Short-term gonadal cultures are sufficient for germline transmission in a songbird.Stem cell reports · 2026Article
- Successful establishment of chicken primordial germ cell (PGC) lines from the endangered Slovakian indigenous chicken breed: the Oravka.Poultry science · 2026Article
- Oxidative homeostasis and survival instincts of chicken egg, embryo and adult.Poultry science · 2026Review
- A practical approach for the stable isolation and cultivation of chicken gonadal primordial germ cells with mitotically inactivated STO feeder cells.Animal bioscience · 2025Article
- Genome Editing in the Chicken: From PGC-Mediated Germline Transmission to Advanced Applications.International journal of molecular sciences · 2025Review
- Article
- RNA-Seq and ATAC-Seq Reveal CYP26A1-Mediated Regulation of Retinoic Acid-Induced Meiosis in Chicken Primordial Germ Cells.Animals : an open access journal from MDPI · 2024Article
- PGC-based cryobanking, regeneration through germline chimera mating, and CRISPR/Cas9-mediated TYRP1 modification in indigenous Chinese chickens.Communications biology · 2024Article
- A Novel, Efficient Method to Isolate Chicken Primordial Germ Cells from Embryonic Blood Using Cell Culture Inserts.Animals : an open access journal from MDPI · 2023Article
- Enhanced cultivation of chicken primordial germ cells.Scientific reports · 2023Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The present study had two aims: (1) To develop a culture system that imitates a normal physiological environment of primordial germ cells (PGCs). There are two types of PGCs in chicken: Circulating blood (cPGCs) and gonadal (gPGCs). The culture condition must support the proliferation of both cPGCs and gPGCs, without affecting their migratory properties and must be deprived of xenobiotic factors, and (2) to propose an easy-to-train, nonlabeling optical technique for the routine identification of live PGCs. To address the first aim, early chicken embryo's feeder cells were examined instead of using feeder cells from mammalian species. The KAv-1 medium at pH 8.0 with the addition of bFGF (basic fibroblast growth factor) was used instead of a conventional culture medium (pH approximately 7.2). Both cPGCs and gPGCs proliferated in vitro and retained their migratory ability after 2 weeks of culture. The cultivated cPGCs and gPGCs colonized the right and/or left gonads of the recipient male and female embryos. To address the second aim, we demonstrated a simple and rapid method to identify live PGCs as bright cells under darkfield illumination. The PGCs rich in lipid droplets in their cytoplasm highly contrasted with the co-cultured feeder layer and other cell populations in the culture.
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