Evidence map›Paper›PMID 33007299›Full record

ArticleContraception2021

Reproductive tract immune cells from pregnant women or those using depot medroxyprogesterone acetate show no excess susceptibility to HIV-1: Results of an ex vivo fusion assay.

Dominika Seidman, Marielle Cavrois, Joan F Hilton, Nadia R Roan, Sarah Averbach, Margaret Takeda, Eric Chang, Nandhini Raman, Ruth Greenblatt, Barbara L Shacklett and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Contraception, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 91% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Dominika SeidmanDepartment of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, USA. Electronic address: Dominika.Seidman@ucsf.edu.
Marielle CavroisGladstone Institute of Virology and Immunology, 1650 Owens St, San Francisco, CA 94158, USA.
Joan F HiltonDepartment of Epidemiology and Biostatistics, University of California San Francisco, San Francisco, CA 94143, USA. Electronic address: Joan.Hilton@ucsf.edu.
Nadia R RoanGladstone Institute of Virology and Immunology, 1650 Owens St, San Francisco, CA 94158, USA; Department of Urology, University of California San Francisco, San Francisco, CA 94143, USA. Electronic address: Nadia.Roan@ucsf.edu.
Sarah AverbachDepartment of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, USA. Electronic address: saverbach@health.ucsd.edu.
Margaret TakedaDepartment of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, USA.
Eric ChangGladstone Institute of Virology and Immunology, 1650 Owens St, San Francisco, CA 94158, USA.
Nandhini RamanGladstone Institute of Virology and Immunology, 1650 Owens St, San Francisco, CA 94158, USA. Electronic address: nandhini.raman@gladstone.ucsf.edu.
Ruth GreenblattDepartment of Epidemiology and Biostatistics, University of California San Francisco, San Francisco, CA 94143, USA; Departments of Clinical Pharmacy and Medicine, University of California San Francisco, San Francisco, CA 94143, USA. Electronic address: Ruth.greenblatt@ucsf.edu.
Barbara L ShacklettDepartment of Medical Microbiology and Immunology, School of Medicine, University of California Davis, Davis, CA 95616, USA. Electronic address: blshacklett@ucdavis.edu.
Karen Smith-McCuneDepartment of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, USA. Electronic address: karen.mccune@ucsf.edu.
University of California, San Francisco · USGladstone Institutes · USUniversity of California, Davis · US

Funding

Virology CoreP30AI027763 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MALLORY O JOHNSON · 1988 to 2026
$93.6M
WOMEN'S REPRODUCTIVE HEALTH RESEARCH CENTERK12HD001262 · NICHD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Andrea Vashti Jackson · 1998 to 2026
$9.3M
UC San Diego Womens Reproductive Health Research ProgramK12HD001259 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CYNTHIA GYAMFI-BANNERMAN · 1999 to 2026
$9.1M
Reproductive Tract Effects of the Intrauterine Device: Implications for HIV RiskR01AI111925 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI SMITH-MCCUNE, KAREN K · 2014 to 2017
$2.5M
NIAID NIH HHS P30 AI027763NIAID NIH HHS R01 AI111925NICHD NIH HHS K12 HD001259NICHD NIH HHS K12 HD001262
6 · The paper itself

Abstract

introductionEx vivo fusion assays offer an efficient method for studying HIV-1 entry associated with contraceptive use and pregnancy outside of cohort studies of HIV-1 incidence.

methodsWe measured ex vivo HIV-1 fusion to cervical or endometrial immune cells from three groups of women: pregnant, non-pregnant not using hormonal or intrauterine contraception, and using depot medroxyprogesterone acetate (DMPA). RESULTS AND

conclusionsThere was no excess susceptibility to HIV-1 fusion of cells from pregnant women or DMPA users compared to controls. Although the number of target cells in endometrium was higher in DMPA users compared to controls, HIV-1 fusion was lower. IMPLICATIONS: In ex vivo assays, HIV-1 showed no enhanced fusion to cervical immune cells from pregnant women or DMPA users compared to controls, and lower fusion to endometrial immune cells from DMPA users. This assay is useful for studying hormonal and contraceptive effects on HIV-1 entry into reproductive tract immune cells.

Indexed as

Contraceptive Agents, FemaleHIV-1Cervix UteriContraceptive AgentsFemaleHumansMedroxyprogesterone AcetatePregnancyPregnant PeopleContraceptive AgentsContraceptive Agents, FemaleMedroxyprogesterone AcetateContraception and HIV-1 riskHIV-1 fusion assayPregnancy and HIV-1 riskReproductive tract HIV-1 target cells

Identifiers

PMID33007299
PMCPMC7736211
OpenAlexW3090204168

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.