ArticlePlant cell reports2021
Unusual DNA-binding properties of the Arabidopsis thaliana WRKY50 transcription factor at target gene promoters.
Article in Plant cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 8 citations in OpenAlex.
- Wounding and Phospholipase C Inhibition: Evaluation of the Alkaloid Profiling in Opium Poppy.Plants (Basel, Switzerland) · 2025Article
- Methodology for Constructing a Knowledgebase for Plant Gene Regulation Information.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Genomic distribution and context dependent functionality of novel WRKY transcription factor binding sites.BMC genomics · 2022Article
- Molecular cloning and expression analysis of a WRKY transcription factor gene,Plant signaling & behavior · 2021Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
key messageWRKY50 from A. thaliana requires WT-boxes at target gene promoters for activation and binding. Based on the genome-wide prediction of WRKY50 target genes and the similarity of a WRKY50 binding site to WT-boxes in microbe-associated molecular pattern (MAMP)-responsive cis-regulatory modules (CRM), four WT-box containing CRMs from the promoter region of three WRKY50 target genes were investigated for their interaction with WRKY50. These target genes are DJ1E, WRKY30 and ATBBE4. Two of the four CRMs, one from DJ1E and one from WRKY30, were able to activate reporter gene expression in the presence of WRKY50. Activation requires the WT-boxes GGACTTTT, GGACTTTG from DJ1E and GGACTTTC from WRKY30. WRKY50 does not activate a second CRM from WRKY30 and the CRM from ATBBE4, both containing the WT-box TGACTTTT. In vitro gel-shift assays demonstrate WT-box-specific binding of the WRKY50 DNA-binding domain to all four CRMs. This work shows a high flexibility of WRKY50 binding site recognition beyond the classic W-box TTGACC/T.
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