Evidence map›Paper›PMID 33005742›Full record

ArticleBioengineering & translational medicine2020

In situ forming microporous gelatin methacryloyl hydrogel scaffolds from thermostable microgels for tissue engineering.

Nicole Zoratto, Donatella Di Lisa, Joseph de Rutte, Md Nurus Sakib, Angelo Roncalli Alves E Silva, Ali Tamayol, Dino Di Carlo, Ali Khademhosseini, Amir Sheikhi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioengineering & translational medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 60 citations in OpenAlex.

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  9. Exosomal Delivery of Interleukin-10 Reduces Infection-Associated Inflammation in a 3D-Printed Model of a Humanized Feto-Maternal Interface.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 3 countries.

Nicole ZorattoDepartment of Bioengineering University of California, Los Angeles Los Angeles California USA.
Donatella Di LisaDepartment of Bioengineering University of California, Los Angeles Los Angeles California USA.
Joseph de RutteDepartment of Bioengineering University of California, Los Angeles Los Angeles California USA.
Md Nurus SakibDepartment of Chemical Engineering The Pennsylvania State University Pennsylvania USA.
Angelo Roncalli Alves E SilvaExperimental Biology Center (NUBEX), University of Fortaleza (UNIFOR) Fortaleza Ceará Brazil.
Ali TamayolUniversity of Connecticut Health Center Farmington Connecticut USA.
Dino Di CarloDepartment of Bioengineering University of California, Los Angeles Los Angeles California USA.
Ali KhademhosseiniDepartment of Bioengineering University of California, Los Angeles Los Angeles California USA.
Amir SheikhiDepartment of Bioengineering University of California, Los Angeles Los Angeles California USA.ORCID https://orcid.org/0000-0002-4495-6675
California NanoSystems Institute · USPennsylvania State University · USUConn Health · USUniversidade de Fortaleza · BRUniversity of California, Los Angeles · US

Funding

Microengineered scaffolds carrying patient-specific cells and growth factors for treatment of volumetric muscle lossR01AR073822 · NIAMS · UNIVERSITY OF NEBRASKA LINCOLN · PI TAMAYOL, ALI · 2018 to 2022
$1.8M
Multifunctional dressing for treatment of diabetic woundsR01GM126831 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI DOKMECI, MEHMET REMZI, TAMAYOL, ALI · 2018 to 2021
$1.3M
NIAMS NIH HHS R01 AR073822NIGMS NIH HHS R01 GM126831
6 · The paper itself

Abstract

Converting biopolymers to extracellular matrix (ECM)-mimetic hydrogel-based scaffolds has provided invaluable opportunities to design in vitro models of tissues/diseases and develop regenerative therapies for damaged tissues. Among biopolymers, gelatin and its crosslinkable derivatives, such as gelatin methacryloyl (GelMA), have gained significant importance for biomedical applications due to their ECM-mimetic properties. Recently, we have developed the first class of in situ forming GelMA microporous hydrogels based on the chemical annealing of physically crosslinked GelMA microscale beads (microgels), which addressed several key shortcomings of bulk (nanoporous) GelMA scaffolds, including lack of interconnected micron-sized pores to support on-demand three-dimensional-cell seeding and cell-cell interactions. Here, we address one of the limitations of in situ forming microporous GelMA hydrogels, that is, the thermal instability (melting) of their physically crosslinked building blocks at physiological temperature, resulting in compromised microporosity. To overcome this challenge, we developed a two-step fabrication strategy in which thermostable GelMA microbeads were produced via semi-photocrosslinking, followed by photo-annealing to form stable microporous scaffolds. We show that the semi-photocrosslinking step (exposure time up to 90 s at an intensity of ~100 mW/cm

Indexed as

GelMA microgelsin situ forming microporous hydrogelsMAP gelsmicrofluidicsmicroporous hydrogelsthermostable GelMA microbeadstissue engineering

Identifiers

PMID33005742
PMCPMC7510466
OpenAlexW3048532146

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.