ArticlePeerJ2020
Establishment and characterization of fantail goldfish fin (FtGF) cell line from goldfish,
Article in PeerJ, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 22 citations in OpenAlex.
- Establishment and longitudinal characterisation of a feeder-free embryonic stem-like cell line (ATES1) derived from blastomeres of the climbing perch, Anabas testudineus.Fish physiology and biochemistry · 2026Article
- Prevalence of cyprinid herpesvirus-2 in goldfish aquarium shops in Tamil Nadu, India: a case report.In vitro cellular & developmental biology. Animal · 2026Article
- Development and characterization of a cell line from the caudal fin of Schizothorax niger (Heckel, 1838) for in vitro toxicity testing.In vitro cellular & developmental biology. Animal · 2026Article
- In Vitro Fish Cell Culture: From Primary Muscle Cells to Cell-Based Meat in Cyprinidae.Biology · 2026Review
- Establishment and Partial Characterization of Three Novel Permanent Cell Lines Originating from European Freshwater Fish Species.Pathogens (Basel, Switzerland) · 2025Article
- Fish cell line: depositories, web resources and future applications.Cytotechnology · 2024Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHerpesviral hematopoietic necrosis disease, caused by cyprinid herpesvirus-2 (CyHV-2), is responsible for massive mortalities in the aquaculture of goldfish,
methodsCaudal fin tissue samples were collected from goldfish without killing the fish. Cell culture of goldfish caudal fin cells was carried out using Leibovitz's L-15 (L-15) medium containing 20% FBS and 1X concentration of antibiotic antimycotic solution, incubated at 28 °C. Cells were characterized and origin of the cells was confirmed by sequencing fragments of the 16S rRNA and COI genes. CyHV-2 was grown in the FtGF cells and passaged continuously 20 times. The infectivity of the CyHV-2 isolated using FtGF cells was confirmed by experimental infection of naïve goldfish.
resultsThe cell line has been passaged up to 56 times in L-15 with 10% FBS. Karyotyping of FtGF cells at 30
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