ArticleScientific reports2020
Full-length transcriptome sequencing analysis and development of EST-SSR markers for the endangered species Populus wulianensis.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed.
- Genetic diversity, population structure, and DNA fingerprinting of Ailanthus altissima var. erythrocarpa based on EST-SSR markers.Scientific reports · 2023Article
- Transcriptome analysis of Haemaphysalis flava female using Illumina HiSeq 4000 sequencing: de novo assembly, functional annotation and discovery of SSR markers.Parasites & vectors · 2023Article
- Transcriptome Analysis Reveals Genes Associated with Flooding Tolerance in Mulberry Plants.Life (Basel, Switzerland) · 2023Article
- Optimization and application of non-native Phragmites australis transcriptome assemblies.PloS one · 2023Article
- Development and validation of SSR markers related to flower color based on full-length transcriptome sequencing in Chrysanthemum.Scientific reports · 2022Article
- Microsatellite analysis and polymorphic marker development based on the full-length transcriptome of Camellia chekiangoleosa.Scientific reports · 2022Article
- Full-length fruit transcriptomes of southern highbush (Vaccinium sp.) and rabbiteye (V. virgatum Ait.) blueberry.BMC genomics · 2022Article
- De novo transcriptome assembly, gene annotation, and EST-SSR marker development of an important medicinal and edible crop, Amomum tsaoko (Zingiberaceae).BMC plant biology · 2022Article
- Pieces of evidence of enhanced cellulose biosynthesis in the low-Cd cultivar and high expression level of transportation genes in the high-Cd cultivar of Lactuca sativa.Environmental science and pollution research international · 2022Article
- De novo transcriptome assembly and analysis of gene expression in different tissues of moth bean (Vigna aconitifolia) (Jacq.) Marechal.BMC plant biology · 2022Article
- Full-length transcriptome sequencing analysis and characterization, development and validation of microsatellite markers inFrontiers in plant science · 2022Article
- Full-length transcriptome analysis ofPeerJ · 2022Article
- Multi-Omics Analysis of Gene and Protein Candidates Possibly Related to Tetrodotoxin Accumulation in the Skin ofMarine drugs · 2021Article
- Transcriptome repository of North-Western Himalayan endangered medicinal herbs: a paramount approach illuminating molecular perspective of phytoactive molecules and secondary metabolism.Molecular genetics and genomics : MGG · 2021Review
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- Developing a new genic SSR primer database in faba bean (Vicia faba L.).Journal of applied genetics · 2021Article
- Article
- Full-length SMRT transcriptome sequencing and microsatellite characterization in Paulownia catalpifolia.Scientific reports · 2021Article
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6 authors.
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Abstract
Populus wulianensis is an endangered species endemic to Shandong Province, China. Despite the economic and ornamental value of this species, few genomics and genetic studies have been performed. In this study, we performed a relevant analysis of the full-length transcriptome sequencing data of P. wulianensis and obtained expressed sequence tag (EST)-simple sequence repeat (SSR) markers with polymorphisms that can be used for further genetic research. In total, 8.18 Gb (3,521,665) clean reads with an average GC content of 42.12% were obtained. From the corrected 64,737 high-quality isoforms, 42,323 transcript sequences were obtained after redundancy analysis with CD-HIT. Among these transcript sequences, 41,876 sequences were annotated successfully. A total of 23,539 potential EST-SSRs were identified from 16,057 sequences. Excluding mononucleotides, the most abundant motifs were trinucleotide SSRs (47.80%), followed by di- (46.80%), tetra- (2.98%), hexa- (1.58%) and pentanucleotide SSRs (0.84%). Among the 100 designed EST-SSRs, 18 were polymorphic with high PIC values (0.721 and 0.683) and could be used for analyses of the genetic diversity and population structure of P. wulianensis. These full-length transcriptome sequencing data will facilitate gene discovery and functional genomics research in P. wulianensis, and the novel EST-SSRs developed in our study will promote molecular-assisted breeding, genetic diversity and conservation biology research in this species.
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