ArticleBMC microbiology2020
Development and comparative validation of genomic-driven PCR-based assays to detect Xanthomonas citri pv. citri in citrus plants.
Article in BMC microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Prevalence ofJCO precision oncology · 2026Article
- Herbarium specimen sequencing allows precise dating of Xanthomonas citri pv. citri diversification history.Nature communications · 2023Article
- MALDI-TOF Mass Spectrometry for the Diagnosis of Citrus Canker Caused byMolecules (Basel, Switzerland) · 2022Article
- Molecular epidemiology of the citrus bacterial pathogenEvolutionary applications · 2022Article
- Using Genomics to Design a Pathovar-Specific Loop-Mediated Isothermal Amplification (LAMP) Assay, for the Improved Detection ofMicroorganisms · 2022Article
- Intra-Laboratory Evaluation of DNA Extraction Methods and Assessment of a Droplet Digital PCR for the Detection of Xanthomonas citri pv. citri on Different Citrus SpeciesInternational journal of molecular sciences · 2022Article
- First historical genome of a crop bacterial pathogen from herbarium specimen: Insights into citrus canker emergence.PLoS pathogens · 2021Article
- Diversity and Geographical Structure ofMicroorganisms · 2021Article
- Trends in Molecular Diagnosis and Diversity Studies for Phytosanitary RegulatedMicroorganisms · 2021Review
- Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens.Frontiers in microbiology · 2021Article
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Authors and funding
13 authors at 6 institutions in 3 countries.
Funding
Abstract
backgroundAsiatic Citrus Canker, caused by Xanthomonas citri pv. citri, severely impacts citrus production worldwide and hampers international trade. Considerable regulatory procedures have been implemented to prevent the introduction and establishment of X. citri pv. citri into areas where it is not present. The effectiveness of this surveillance largely relies on the availability of specific and sensitive detection protocols. Although several PCR- or real-time PCR-based methods are available, most of them showed analytical specificity issues. Therefore, we developed new conventional and real-time quantitative PCR assays, which target a region identified by comparative genomic analyses, and compared them to existing protocols.
resultsOur assays target the X. citri pv. citri XAC1051 gene that encodes for a putative transmembrane protein. The real-time PCR assay includes an internal plant control (5.8S rDNA) for validating the assay in the absence of target amplification. A receiver-operating characteristic approach was used in order to determine a reliable cycle cut-off for providing accurate qualitative results. Repeatability, reproducibility and transferability between real-time devices were demonstrated for this duplex qPCR assay (XAC1051-2qPCR). When challenged with an extensive collection of target and non-target strains, both assays displayed a high analytical sensitivity and specificity performance: LOD
conclusionsWe developed new valuable detection assays useful for routine diagnostics and surveillance of X. citri pv. citri in citrus material. Their reliability was evidenced through numerous trials on a wide range of bacterial strains and plant samples. Successful detection of the pathogen was achieved from both artificially and naturally infected plants, as well as from citrus herbarium samples, suggesting that these assays will have positive impact both for future applied and academic research on this bacterium.
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