ArticleThe Journal of surgical research2021
Small Bowel Resection Increases Paracellular Gut Barrier Permeability via Alterations of Tight Junction Complexes Mediated by Intestinal TLR4.
Article in The Journal of surgical research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Cellular senescence in human liver under normal aging and cancer.Cell genomics · 2026Article
- Liver injury in a preclinical model of short bowel syndrome is associated with impaired autophagy.Scientific reports · 2025Article
- Curcumin/piperine modulates the butyrate levels and alleviates diabetic kidney disease via Nrf2/HO-1 signaling pathway.Renal failure · 2025Article
- Xenotransplanted human organoids identify transepithelial zinc transport as a key mediator of intestinal adaptation.Nature communications · 2024Article
- Intestinal Knockout of Peroxisome Proliferator-Activated Receptor-Alpha Affects Structural Adaptation but not Liver Injury Following Massive Enterectomy.Journal of pediatric surgery · 2023Article
- Disruption of Enterohepatic Circulation of Bile Acids Ameliorates Small Bowel Resection Associated Hepatic Injury.Journal of pediatric surgery · 2023Article
- A novel maladaptive unfolded protein response as a mechanism for small bowel resection-induced liver injury.American journal of physiology. Gastrointestinal and liver physiology · 2022Article
- Distal Small Bowel Resection Yields Enhanced Intestinal and Colonic Adaptation.The Journal of surgical research · 2022Article
- The role and mechanism of gut microbiota-derived short-chain fatty in the prevention and treatment of diabetic kidney disease.Frontiers in immunology · 2022Review
- Modified Renshen Wumei Decoction Alleviates Intestinal Barrier Destruction in Rats with Diarrhea.Journal of microbiology and biotechnology · 2021Article
- Liver injury after small bowel resection is prevented in obesity-resistant 129S1/SvImJ mice.American journal of physiology. Gastrointestinal and liver physiology · 2021Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundShort bowel syndrome resulting from small bowel resection (SBR) is associated with significant morbidity and mortality. Many adverse sequelae including steatohepatitis and bacterial overgrowth are thought to be related to increased bacterial translocation, suggesting alterations in gut permeability. We hypothesized that after intestinal resection, the intestinal barrier is altered via toll-like receptor 4 (TLR4) signaling at the intestinal level.
methodsB6 and intestinal-specific TLR4 knockout (iTLR4 KO) mice underwent 50% SBR or sham operation. Transcellular permeability was evaluated by measuring goblet cell associated antigen passages via two-photon microscopy. Fluorimetry and electron microscopy evaluation of tight junctions (TJ) were used to assess paracellular permeability. In parallel experiments, single-cell RNA sequencing measured expression of intestinal integral TJ proteins. Western blot and immunohistochemistry confirmed the results of the single-cell RNA sequencing.
resultsThere were similar number of goblet cell associated antigen passages after both SBR and sham operation (4.5 versus 5.0, P > 0.05). Fluorescein isothiocyanate-dextran uptake into the serum after massive SBR was significantly increased compared with sham mice (2.13 ± 0.39 ng/μL versus 1.62 ± 0.23 ng/μL, P < 0.001). SBR mice demonstrated obscured TJ complexes on electron microscopy. Single-cell RNA sequencing revealed a decrease in TJ protein occludin (21%) after SBR (P < 0.05), confirmed with immunostaining and western blot analysis. The KO of iTLR4 mitigated the alterations in permeability after SBR.
conclusionsPermeability after SBR is increased via changes at the paracellular level. However, these alterations were prevented in iTLR4 mice. These findings suggest potential protein targets for restoring the intestinal barrier and obviating the adverse sequelae of short bowel syndrome.
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