ArticlePLoS pathogens2020
The C4 protein encoded by tomato leaf curl Yunnan virus reverses transcriptional gene silencing by interacting with NbDRM2 and impairing its DNA-binding ability.
Article in PLoS pathogens, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Characterization of the C4 protein encoded by parsley yellow leaf curl virus.Virology journal · 2026Article
- Small RNA mobility and plant virus diseases.Journal of experimental botany · 2026Review
- Epigenetic and Epitranscriptomic Antiviral Responses in Plants for Disease Management.Viruses · 2025Review
- Two viral proteins translated from one open reading frame target different layers of plant defense.Plant communications · 2024Article
- IAA-miR164a-NAC100L1 module mediates symbiotic incompatibility of cucumber/pumpkin grafted seedlings through regulating callose deposition.Horticulture research · 2024Article
- Transcriptional and epigenetic changes during tomato yellow leaf curl virus infection in tomato.BMC plant biology · 2023Article
- Broad functional landscape of geminivirus-encoded C4/AC4 protein.Crop health · 2023Review
- Review
- Plant resistance to tomato yellow leaf curl virus is enhanced byFrontiers in microbiology · 2023Article
- DNA methylation dynamics in response to abiotic and pathogen stress in plants.Plant cell reports · 2022Review
- Article
- Turnip mosaic virus manipulates DRM2 expression to regulate host CHH and CHG methylation for robust infection.Stress biology · 2022Article
- The novel C5 protein from tomato yellow leaf curl virus is a virulence factor and suppressor of gene silencing.Stress biology · 2022Article
- Insights into the multifunctional roles of geminivirus-encoded proteins in pathogenesis.Archives of virology · 2022Review
- Geminiviruses employ host DNA glycosylases to subvert DNA methylation-mediated defense.Nature communications · 2022Article
- Article
- Phosphorylation of plant virus proteins: Analysis methods and biological functions.Frontiers in microbiology · 2022Review
- CsbZIP2-miR9748-CsNPF4.4 Module Mediates High Temperature Tolerance of Cucumber Through Jasmonic Acid Pathway.Frontiers in plant science · 2022Article
- Small but mighty: Functional landscape of the versatile geminivirus-encoded C4 protein.PLoS pathogens · 2021Review
- Impact of viral silencing suppressors on plant viral synergism: a global agro-economic concern.Applied microbiology and biotechnology · 2021Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, cytosine DNA methylation is an efficient defense mechanism against geminiviruses, since methylation of the viral genome results in transcriptional gene silencing (TGS). As a counter-defense mechanism, geminiviruses encode viral proteins to suppress viral DNA methylation and TGS. However, the molecular mechanisms by which viral proteins contribute to TGS suppression remain incompletely understood. In this study, we found that the C4 protein encoded by tomato leaf curl Yunnan virus (TLCYnV) suppresses methylation of the viral genome through interacting with and impairing the DNA-binding ability of NbDRM2, a pivotal DNA methyltransferase in the methyl cycle. We show that NbDRM2 catalyzes the addition of methyl groups on specific cytosine sites of the viral genome, hence playing an important role in anti-viral defense. Underscoring the relevance of the C4-mediated suppression of NbDRM2 activity, plants infected by TLCYnV producing C4(S43A), a point mutant version of C4 unable to interact with NbDRM2, display milder symptoms and lower virus accumulation, concomitant with enhanced viral DNA methylation, than plants infected by wild-type TLCYnV. Expression of TLCYnV C4, but not of the NbDRM2-interaction compromised C4(S43A) mutant, in 16c-TGS Nicotiana benthamiana plants results in the recovery of GFP, a proxy for suppression of TGS. This study provides new insights into the molecular mechanisms by which geminiviruses suppress TGS, and uncovers a new viral strategy based on the inactivation of the methyltransferase NbDRM2.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.