ArticlePloS one2020
Genotyping of Giardia duodenalis in children in upper Egypt using assemblage- specific PCR technique.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 2 of them syntheses that pooled it.
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Who cites it
18 citing papers in PubMed, 2 syntheses or guidelines pooled it, 42 citations in OpenAlex.
- Molecular Epidemiology of Giardia Species in Different Hosts in Africa: A Systematic Review and Meta-Analysis.Acta parasitologica · 2026Pooled it
- A 'One Health' perspective of Africa-wide distribution and prevalence of Giardia species in humans, animals and waterbodies: a systematic review and meta-analysis.Parasitology · 2023Pooled it
- Parasitic diseases of marine fishes and their impact on human health: focus on the Egyptian biosphere: A review.Veterinary research communications · 2026Review
- Multilocus Oxford Nanopore sequencing reveals genetic diversity of Giardia duodenalis and Cryptosporidium spp. in surface and wastewater in Southwestern Colombia.Parasites & vectors · 2026Article
- Evaluating the role of synanthropic filth flies in the transmission of zoonotic parasites: field and laboratory evidence from different animal rearing sites in upper Egypt with focus on Cryptosporidium spp.BMC veterinary research · 2025Article
- Prevalence and Genetic Diversity ofJournal of parasitology research · 2025Review
- Molecular characterization and risk analysis of Giardia duodenalis assemblages in corticosteroid-treated and non-treated patients in Ismailia, Arab Republic of Egypt.Gut pathogens · 2024Article
- Assemblage characterization of Giardia duodenalis in South Khorasan province, eastern Iran, using HRM real-time PCR method.Molecular biology reports · 2024Article
- Microscopy detection and molecular characterisation ofFrontiers in public health · 2024Article
- Prevalence and assemblage of Giardia duodenalis in a case-control study of children under 5 years from Jimma, Southwest Ethiopia.Parasitology research · 2023Article
- Genotyping and epidemiological distribution of diarrhea-causing isolates of Giardia duodenalis in southeastern part of West Bengal, India.Parasitology research · 2023Article
- Prevalence and molecular characterization ofVeterinary world · 2023Article
- A shotgun metagenomic analysis of the fecal microbiome in humans infected with Giardia duodenalis.Parasites & vectors · 2023Article
- Association Between Giardia Genotype and Oxidative Stress Biomarkers Among Giardia-Infected Children: A Case-Control Study.Acta parasitologica · 2022Article
- Prevalence and multi-locus genotyping of Giardia duodenalis in rabbits from Shaanxi province in northwestern China.Parasite (Paris, France) · 2021Article
- Molecular Genotyping ofIranian journal of parasitologyArticle
- Comparison ofTropical parasitologyArticle
- Molecular detection and genotyping ofTropical parasitologyArticle
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Giardia duodenalis is a common gastrointestinal protozoan parasite, causing diarrheal illness in humans worldwide. Yet, the distribution of G. duodenalis genotypes among human patients and their clinical relevance remains controversial. This study aimed to detect G. duodenalis in children in Upper Egypt and identify causative genotypes and elucidate a possible correlation between genotype and clinical presentation. One hundred sixty-five children, regardless of symptoms, were tested for giardiasis. Giardia positive stool samples (40/165) were subjected to PCR amplification targeting the tpi gene with positive PCR results in only 35 cases (87.5%). Assemblage-specific amplification of genotypes (A, B, and the zoonotic E strains) revealed predominantly G. duodenalis Assemblage A (45.7%). Assemblage B and mixed A and B infections were detected in 31.4% and 22.8% of children, respectively. Assemblage E was not detected. G. duodenalis assemblage A was dominant in children who complained of diarrhea and abdominal cramps. In contrast, asymptomatic children with positive stool samples display a higher frequency of assemblage B and mixed infections. The study highlights the predominance of Giardia Assemblage A in our study locality. This study is the first for this endemic area to use the copro-PCR technique for diagnosis and genotyping of giardiasis. Study results show the value of simple species-specific primers for genotyping in communities with little access to laboratory resources. Further genetic studies are needed to clarify the association between parasite genetic diversity and patient symptomatology.
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