ArticlePlant biotechnology journal2021
Precision genome editing in plants via gene targeting and subsequent break-induced single-strand annealing.
Article in Plant biotechnology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Defect-complementation homologous recombination: A novel strategy for precise genome engineering of virulent phages.Synthetic and systems biotechnology · 2026Article
- The evolving landscape of precise DNA insertion in plants.Nature communications · 2025Review
- Breeding herbicide-resistant rice using CRISPR-Cas gene editing and other technologies.Plant communications · 2025Review
- A dual-function selection system enables positive selection of multigene CRISPR mutants and negative selection ofaBIOTECH · 2024Article
- Synthetic directed evolution for targeted engineering of plant traits.Frontiers in plant science · 2024Review
- Transcriptionally linked simultaneous overexpression of P450 genes for broad-spectrum herbicide resistance.Plant physiology · 2023Article
- Targeted deletion of grape retrotransposon associated with fruit skin color via CRISPR/Cas9 in Vitis labrascana 'Shine Muscat'.PloS one · 2023Article
- Metabolic pathway genes for editing to enhance multiple disease resistance in plants.Journal of plant research · 2022Article
- Genome Editing Technology and Its Application to Metabolic Engineering in Rice.Rice (New York, N.Y.) · 2022Review
- Advances in Delivery Mechanisms of CRISPR Gene-Editing Reagents in Plants.Frontiers in genome editing · 2022Review
- Sequence modification on demand: search and replace tools for precise gene editing in plants.Transgenic research · 2021Review
- Precision genome editing in plants via gene targeting and subsequent break-induced single-strand annealing.Plant biotechnology journal · 2021Article
- Precise Genome Editing in miRNA Target Site via Gene Targeting and Subsequent Single-Strand-Annealing-Mediated Excision of the Marker Gene in Plants.Frontiers in genome editing · 2020Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome editing via artificial nucleases such as CRISPR/Cas9 has become popular in plants now. However, small insertions or deletions are major mutations and nucleotide substitutions rarely occur when DNA cleavage is induced. To induce nucleotide substitutions, a base editor utilizing dead or nickase-type Cas9 fused with deaminase have been developed. However, the direction and position of practical substitution are still limited. In this context, homologous recombination (HR)-mediated gene targeting (GT) has advantages because any mutations existing on the donor DNA are copied and passed onto the endogenous DNA. As HR-mediated GT is extremely rare in higher plants, positive-negative selection has been used to isolate cells in which GT has occurred. After successful selection, positive selection marker is no longer needed and should ideally be eliminated. In a previous study, we reported a seamless piggyBac-transposon-mediated marker elimination system. Precision marker elimination efficiency in this system is very high. The piggyBac transposon integrates into the host genome at TTAA elements and excises without leaving a footprint at the excised site, so a TTAA sequence is necessary at the location of a positive selection marker. To compensate for this limitation, we have developed a novel marker elimination system using an I-SceI break and subsequent single-strand annealing (SSA)-mediated DNA repair system.
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