Article3 Biotech2020
Two-step method for isolation of high-quality RNA from stored seeds of maize rich in starch.
Article in 3 Biotech, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 28 citations in OpenAlex.
- Transcriptomic Analysis of Cold-Induced Temporary Cysts in Marine DinoflagellateInternational journal of molecular sciences · 2025Article
- Transcriptomic response of marine dinoflagellate Prorocentrum cordatum to phosphorus deficiency.Scientific reports · 2025Article
- Harnessing the potential of millets for climate-resilient and sustainable agriculture.Frontiers in plant science · 2025Review
- Combined Metabolome and Transcriptome Analyses of Maize Leaves Reveal Global Effect of Biochar on Mechanisms Involved in Anti-Herbivory toMetabolites · 2024Article
- Exploring novel SNPs and candidate genes associated with seed allometry inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Removal of the large inverted repeat from the plastid genome reveals gene dosage effects and leads to increased genome copy number.Nature plants · 2024Article
- Article
- Expression analysis of3 Biotech · 2021Article
- Low expression of carotenoids cleavage dioxygenase 1 (ccd1) gene improves the retention of provitamin-A in maize grains during storage.Molecular genetics and genomics : MGG · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A modified SDS-Trizol method was optimized for isolation of total RNA from the stored maize seeds at regular interval of one month for 4 months. Use of SDS extraction buffer before the use of Trizol reduced the co-precipitation problem associated with high carbohydrate content in the seed. Recorded mean RNA yield from seeds across the storage intervals was 978.6 ± 65.46 ng/µl. Average spectrophotometric values (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.