ArticleScientific reports2020
Next-generation sequencing in the diagnosis of viral encephalitis: sensitivity and clinical limitations.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Article
- Combined NGS and proteomics improves viral diagnostics in wildlife: coronaviruses confirmed in hedgehogs.Frontiers in cellular and infection microbiology · 2026Article
- Development of a semicomprehensive detection method for paramyxoviruses and its validation using Indonesian bats.Scientific reports · 2025Article
- Enhancing bloodstream infection diagnostics: a novel filtration and targeted next-generation sequencing approach for precise pathogen identification.Frontiers in microbiology · 2025Article
- Exploring the appropriate situation of performing CSF mNGS in patients with proposed intracranial infections.BMC neurology · 2024Article
- Differentiation between viral and autoimmune limbic encephalitis: a prospective cohort study with development and validation of a diagnostic model.Journal of neurology · 2024Observational
- Understanding the Neurotrophic Virus Mechanisms and Their Potential Effect on Systemic Lupus Erythematosus Development.Brain sciences · 2024Review
- Elevated phospholipids and acylcarnitines C4 and C5 in cerebrospinal fluid distinguish viral CNS infections from autoimmune neuroinflammation.Journal of translational medicine · 2023Article
- Metagenomic search of viral coinfections in herpes simplex encephalitis patients.Journal of neurovirology · 2023Article
- Epidemiology and Transmission Dynamics of Viral Encephalitides in West Africa.Infectious disease reports · 2023Review
- The central nervous system is a potential reservoir and possible origin of drug resistance in hepatitis B infection.Journal of virus eradication · 2023Article
- Early autoimmunity and outcome in virus encephalitis: a retrospective study based on tissue-based assay.Journal of neurology, neurosurgery, and psychiatry · 2023Observational
- Review
- Plasma virome dynamics in chronic hepatitis B virus infected patients.Frontiers in microbiology · 2023Article
- Clinical application and drug-use-guidance value of metagenomic next-generation sequencing in central nervous system infection.American journal of translational research · 2023Article
- Application of metagenomic next-generation sequencing in the diagnosis of infectious diseases of the central nervous system after empirical treatment.World journal of clinical cases · 2022Article
- Patients with Infections of The Central Nervous System Have Lowered Gut Microbiota Alpha Diversity.Current issues in molecular biology · 2022Article
- Hepatitis B virus in cerebrospinal fluid of a patient with purulent bacterial meningitis detected by multiplex-PCR: A case report.World journal of clinical cases · 2022Article
- Viral RNA Metagenomics ofViruses · 2021Article
- Contamination Issue in Viral Metagenomics: Problems, Solutions, and Clinical Perspectives.Frontiers in microbiology · 2021Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Identification of pathogens causing viral encephalitis remains challenging, and in over 50% of cases the etiologic factor remains undetermined. Next-generation sequencing (NGS) based metagenomics has been successfully used to detect novel and rare infections, but its value for routine diagnosis of encephalitis remains unclear. The aim of the present study was to determine the sensitivity of shotgun metagenomic sequencing protocols, which include preamplification, and testing it against cerebrospinal fluid (CSF) samples from encephalitis patients. For sensitivity testing HIV and HBV positive sera were serially diluted in CSF from an uninfected patient. NGS repeatedly detected HIV and HBV sequences present at concentrations from 10
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