ArticleScientific reports2020
Development of droplet digital PCR for the detection of Tilletia laevis, which causes common bunt of wheat, based on the SCAR marker derived from ISSR and real-time PCR.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 25 citations in OpenAlex.
- Alfalfa Rotation Enhances Soil Microbial Diversity and Reduces Tilletia foetida DNA Abundance in Common Bunt-Infected Wheat Fields.The plant pathology journal · 2026Article
- Research progress on nucleic acid amplification-based detection technologies for phytopathogenic fungi.Applied microbiology and biotechnology · 2026Review
- Sustainable microbial solutions for managing fungal threats in wheat: progress and future directions.World journal of microbiology & biotechnology · 2025Review
- Recent developments in waterborne pathogen detection technologies.Environmental monitoring and assessment · 2025Review
- Transcriptome analysis of different developmental stages ofCurrent research in microbial sciences · 2025Article
- Early Detection of BothInternational journal of molecular sciences · 2024Article
- Analytical and clinical validation of multiplex droplet digital PCR assay for detecting pathogenic fungal infection in lungs.Mycology · 2024Article
- Molecular diagnostic assay for pre-harvest detection ofFrontiers in microbiology · 2023Article
- Article
- Development of real-time PCR and droplet digital PCR based marker for the detection ofFrontiers in plant science · 2022Article
- Transcriptomics Analysis of Wheat Tassel Response toFrontiers in plant science · 2022Article
- Metal Organic Frameworks as Biosensing Materials for COVID-19.Cellular and molecular bioengineering · 2021Review
- A Duplex-Droplet Digital PCR Assay for Simultaneous Quantitative Detection ofFrontiers in microbiology · 2021Article
- Digital PCR: What Relevance to Plant Studies?Biology · 2020Review
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9 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Common bunt of wheat caused by Tilletia laevis and/or T. caries (syn. T. tritici), is a major disease in wheat-growing regions worldwide that could lead to 80% or even total loss of production. Even though T. laevis can be distinguished from T. caries on the bases of morphology of teliospores using microscopy technique. However, molecular methods could serve as an additional method to quantify the pathogen. To develop a rapid diagnostic and quantify method, we employed the ISSR molecular marker for T. laevis in this study. The primer ISSR857 generated a polymorphic pattern displaying a 1385 bp T. laevis-specific DNA fragment. A pair of specific primers (L57F/L57R) was designed to amplify a sequence-characterized amplified region (SCAR) (763 bp) for the PCR detection assay. The primers amplified the DNA fragment in the tested isolates of T. laevis but failed in the related species, including T. caries. The detection limit of the primer set (L57F/L57R) was 5 ng/µl of DNA extracted from T. laevis teliospores. A SYBR Green I real-time PCR method for detecting T. laevis with a 100 fg/µl detection limit and droplet digital PCR with a high sensitivity (30 fg/µl detection limit) were developed; this technique showed the most sensitive detection compared to the SCAR marker and SYBR Green I real-time PCR. Additionally, this is the first study related the detection of T. laevis with the droplet digital PCR method.
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