Evidence map›Paper›PMID 32999356›Full record

ArticleScientific reports2020

Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high resolution scanning electron microscopy.

Lucio Ayres Caldas, Fabiana Avila Carneiro, Luiza Mendonça Higa, Fábio Luiz Monteiro, Gustavo Peixoto da Silva, Luciana Jesus da Costa, Edison Luiz Durigon, Amilcar Tanuri, Wanderley de Souza

Abstract read
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed.

  1. Article
  2. Nonlytic Egress and Transmission in the Virus World.Annual review of biochemistry · 2025
    Review
  3. Article
  4. A role for tunneling nanotubes in virus spread.Frontiers in microbiology · 2024
    Review
  5. Article
  6. Tunneling Nanotubes: The Cables for Viral Spread and Beyond.Results and problems in cell differentiation · 2024
    Review
  7. Intercellular Highways in Transport Processes.Results and problems in cell differentiation · 2024
    Review
  8. Understanding lifetime and dispersion of cough-emitted droplets in air.Indoor + built environment : the journal of the International Society of the Built Environment · 2023
    Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lucio Ayres CaldasLaboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Cidade Universitária. Av., Carlos Chagas Filho 373, Prédio CCS, Bloco C, subsolo, CEP: 21941902, Rio de Janeiro, RJ, Brazil. lucio@biof.ufrj.br.
Fabiana Avila CarneiroNúcleo Multidisciplinar de Pesquisa UFRJ-Xerém em Biologia - NUMPEX-BIO, Universidade Federal Do Rio de Janeiro, Campus Duque de Caxias Geraldo Cidade. CEP: 25265-970, Rio de Janeiro, RJ, Brazil.
Luiza Mendonça HigaDepartamento de Genética, Instituto de Biologia, Universidade Federal Do Rio de Janeiro, Rio de Janeiro, Brazil.
Fábio Luiz MonteiroDepartamento de Genética, Instituto de Biologia, Universidade Federal Do Rio de Janeiro, Rio de Janeiro, Brazil.
Gustavo Peixoto da SilvaDepartamento de Virologia, Instituto de Microbiologia Paulo de Góes, Universidade Federal Do Rio de Janeiro, Rio de Janeiro, Brazil.
Luciana Jesus da CostaDepartamento de Virologia, Instituto de Microbiologia Paulo de Góes, Universidade Federal Do Rio de Janeiro, Rio de Janeiro, Brazil.
Edison Luiz DurigonInstituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.
Amilcar TanuriDepartamento de Genética, Instituto de Biologia, Universidade Federal Do Rio de Janeiro, Rio de Janeiro, Brazil.
Wanderley de SouzaLaboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Cidade Universitária. Av., Carlos Chagas Filho 373, Prédio CCS, Bloco C, subsolo, CEP: 21941902, Rio de Janeiro, RJ, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

SARS-CoV-2 is the cause of the ongoing COVID-19 pandemic. Here, we investigated the interaction of this new coronavirus with Vero cells using high resolution scanning electron microscopy. Surface morphology, the interior of infected cells and the distribution of viral particles in both environments were observed 2 and 48 h after infection. We showed areas of viral processing, details of vacuole contents, and viral interactions with the cell surface. Intercellular connections were also approached, and viral particles were adhered to these extensions suggesting direct cell-to-cell transmission of SARS-CoV-2.

Indexed as

AnimalsBetacoronavirusCell LineChlorocebus aethiopsCoronavirus InfectionsCOVID-19Host-Pathogen InteractionsHumansMicroscopy, Electron, ScanningPandemicsPneumonia, ViralSARS-CoV-2Vero Cells

Identifiers

PMID32999356
PMCPMC7528159

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.