ArticleScientific reports2020
Simultaneous TALEN-mediated knockout of chrysanthemum DMC1 genes confers male and female sterility.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Investigation of mechanisms underlying seedlessness in a Sangiovese somatic variant through comparative genomic and histological analyses.BMC plant biology · 2025Article
- Directed mutagenesis in fruit crops.3 Biotech · 2025Review
- Developing a UV-visible reporter-assisted CRISPR/Cas9 gene editing system to alter flowering time in Chrysanthemum indicum.Plant biotechnology journal · 2023Article
- Overcoming Difficulties in Molecular Biological Analysis through a Combination of Genetic Engineering, Genome Editing, and Genome Analysis in HexaploidPlants (Basel, Switzerland) · 2023Review
- Excision of DNA fragments with thePlant biotechnology (Tokyo, Japan) · 2023Article
- Integrated Analysis of microRNA and RNA-Seq Reveals Phenolic Acid Secretion Metabolism in Continuous Cropping ofPlants (Basel, Switzerland) · 2023Article
- Current insights and advances into plant male sterility: new precision breeding technology based on genome editing applications.Frontiers in plant science · 2023Review
- Article
- Flavonoids - flowers, fruit, forage and the future.Journal of the Royal Society of New Zealand · 2023Review
- Combination of long-read and short-read sequencing provides comprehensive transcriptome and new insight for Chrysanthemum morifolium ray-floret colorization.Scientific reports · 2022Article
- Towards the Improvement of Ornamental Attributes in Chrysanthemum: Recent Progress in Biotechnological Advances.International journal of molecular sciences · 2022Review
- Random mutagenesis in vegetatively propagated crops: opportunities, challenges and genome editing prospects.Molecular biology reports · 2022Article
- A chromosome-level genome sequence of Chrysanthemum seticuspe, a model species for hexaploid cultivated chrysanthemum.Communications biology · 2021Article
- Phenotypic Characterization and RT-qPCR Analysis of Flower Development in FPlants (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome editing has become one of the key technologies for plant breeding. However, in polyploid species such as chrysanthemum, knockout of all loci of multiple genes is needed to eliminate functional redundancies. We identified six cDNAs for the CmDMC1 genes involved in meiotic homologous recombination in chrysanthemum. Since all six cDNAs harbored a homologous core region, simultaneous knockout via TALEN-mediated genome editing should be possible. We isolated the CmDMC1 loci corresponding to the six cDNAs and constructed a TALEN-expression vector bearing a CmDMC1 target site containing the homologous core region. After transforming two chrysanthemum cultivars with the TALEN-expression vector, seven lines exhibited disruption of all six CmDMC1 loci at the target site as well as stable male and female sterility at 10-30 °C. This strategy to produce completely sterile plants could be widely applicable to prevent the risk of transgene flow from transgenic plants to their wild relatives.
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