ArticleProceedings of the National Academy of Sciences of the United States of America2020
Three-dimensional digital PCR through light-sheet imaging of optically cleared emulsion.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Cancer and Aging Biomarkers: Classification, Early Detection Technologies and Emerging Research Trends.Biosensors · 2025Review
- Nanocomposite-based PCR reactors to enhance thermal rate and fluorescence intensity in hand-held qPCR device.Journal of nanobiotechnology · 2025Article
- Lossless single-molecule counting to absolute quantify proteoforms.Scientific reports · 2025Article
- Large-scale high-throughput 3D culture, imaging, and analysis of cell spheroids using microchip-enhanced light-sheet microscopy.Biomedical optics express · 2023Article
- Next-Generation Digital Polymerase Chain Reaction: High-Dynamic-Range Single-Molecule DNA Counting via Ultrapartitioning.Analytical chemistry · 2022Article
- Accurate Bulk Quantitation of Droplet Digital Polymerase Chain Reaction.Analytical chemistry · 2021Article
- A CRISPR/Cas9 eraser strategy for contamination-free PCR end-point detection.Biotechnology and bioengineering · 2021Article
- Accurate bulk quantitation of droplet digital PCR.bioRxiv : the preprint server for biology · 2021Article
- A Double-Deck Self-Digitization Microfluidic Chip for Digital PCR.Micromachines · 2020Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The realization of the vast potential of digital PCR (dPCR) to provide extremely accurate and sensitive measurements in the clinical setting has thus far been hindered by challenges such as assay robustness and high costs. Here we introduce a lossless and contamination-free dPCR technology, termed CLEAR-dPCR, which addresses these challenges by completing the dPCR sample preparation, PCR, and readout all in one tube. Optical clearing of the droplet dPCR emulsion was combined with emerging light-sheet fluorescence microscopy, to acquire a three-dimensional (3D) image of a half million droplets sealed in a tube in seconds. CLEAR-dPCR provides ultrahigh-throughput readout results in situ and fundamentally eliminates the possibility of either sample loss or contamination. This approach exhibits improved accuracy over existing dPCR platforms and enables a greatly increased dynamic range to be comparable to that of real-time quantitative PCR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.