Evidence map›Paper›PMID 32999061›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2020

Myristate can be used as a carbon and energy source for the asymbiotic growth of arbuscular mycorrhizal fungi.

Yuta Sugiura, Rei Akiyama, Sachiko Tanaka, Koji Yano, Hiromu Kameoka, Shiori Marui, Masanori Saito, Masayoshi Kawaguchi, Kohki Akiyama, Katsuharu Saito

Open access · greenAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.

0numbers the graph read from it
0cells of the map it votes in
52citing papers in PubMed
16.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

52 citing papers in PubMed, 115 citations in OpenAlex.

  1. Review
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  5. Asymbiotic Growth of Arbuscular Mycorrhizal Fungi.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
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  7. Article
  8. Review
  9. Article
  10. Review
  11. Article
  12. Article
  13. Review
  14. Arbuscular mycorrhizal fungi andFrontiers in plant science · 2025
    Article
  15. Review
  16. Evolution of small molecule-mediated regulation of arbuscular mycorrhiza symbiosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024
    Review
  17. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Yuta SugiuraGraduate School of Science and Technology, Shinshu University, Minamiminowa, 399-4598 Nagano, Japan.ORCID 0000-0003-4948-4950
Rei AkiyamaGraduate School of Science and Technology, Shinshu University, Minamiminowa, 399-4598 Nagano, Japan.
Sachiko TanakaDivision of Symbiotic Systems, National Institute for Basic Biology, Okazaki, 444-0867 Aichi, Japan.ORCID 0000-0002-1746-7921
Koji YanoDivision of Symbiotic Systems, National Institute for Basic Biology, Okazaki, 444-0867 Aichi, Japan.ORCID 0000-0002-4322-3525
Hiromu KameokaGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, 599-8531 Osaka, Japan.ORCID 0000-0002-7246-2242
Shiori MaruiGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, 599-8531 Osaka, Japan.
Masanori SaitoField Science Center, Graduate School of Agricultural Science, Tohoku University, Miyagi, 989-6711 Osaki, Japan.ORCID 0000-0003-4692-9379
Masayoshi KawaguchiDivision of Symbiotic Systems, National Institute for Basic Biology, Okazaki, 444-0867 Aichi, Japan.ORCID 0000-0002-1032-6261
Kohki AkiyamaGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, 599-8531 Osaka, Japan.ORCID 0000-0002-9171-9058
Katsuharu SaitoFaculty of Agriculture, Shinshu University, Minamiminowa, 399-4598 Nagano, Japan saitok@shinshu-u.ac.jp.ORCID 0000-0003-2557-1726
National Institute for Basic Biology · JPOsaka Prefecture University · JPShinshu University · JPTohoku University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Arbuscular mycorrhizal (AM) fungi, forming symbiotic associations with land plants, are obligate symbionts that cannot complete their natural life cycle without a host. The fatty acid auxotrophy of AM fungi is supported by recent studies showing that lipids synthesized by the host plants are transferred to the fungi, and that the latter lack genes encoding cytosolic fatty acid synthases. Therefore, to establish an asymbiotic cultivation system for AM fungi, we tried to identify the fatty acids that could promote biomass production. To determine whether AM fungi can grow on medium supplied with fatty acids or lipids under asymbiotic conditions, we tested eight saturated or unsaturated fatty acids (C12 to C18) and two β-monoacylglycerols. Only myristate (C14:0) led to an increase in the biomass of

Indexed as

CarbonCell WallEnergy MetabolismGlomeromycotaHyphaeMycorrhizaeMyristatesCarbonMyristatesfatty acid auxotrophyimmobilized cell culturemycorrhizal symbiosispure culture

Identifiers

PMID32999061
PMCPMC7568319
OpenAlexW3090219007

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.