Evidence map›Paper›PMID 32998338›Full record

ArticleCancers2020

Efficient Propagation of Circulating Tumor Cells: A First Step for Probing Tumor Metastasis.

Jerry Xiao, Joseph R McGill, Kelly Stanton, Joshua D Kassner, Sujata Choudhury, Richard Schlegel, Zuben E Sauna, Paula R Pohlmann, Seema Agarwal

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancers, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.3field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 27 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Jerry XiaoLombardi Cancer Center, Georgetown University Medical Center, Washington, DC 20007, USA.ORCID 0000-0001-8315-1178
Joseph R McGillHemostasis Branch, Division of Plasma Protein Therapeutics, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD 20993, USA.
Kelly StantonDepartment of Pathology, Yale University, New Haven, CT 06511, USA.ORCID 0000-0002-0707-5457
Joshua D KassnerDepartment of Medicine, Medstar Hospital, Georgetown University Medical Center, Washington, DC 20007, USA.ORCID 0000-0002-8774-7638
Sujata ChoudhuryDepartment of Pathology, Center for Cell Reprogramming, Georgetown University Medical Center, Washington, DC 20007, USA.
Richard SchlegelDepartment of Pathology, Center for Cell Reprogramming, Georgetown University Medical Center, Washington, DC 20007, USA.
Zuben E SaunaHemostasis Branch, Division of Plasma Protein Therapeutics, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD 20993, USA.
Paula R PohlmannLombardi Cancer Center, Georgetown University Medical Center, Washington, DC 20007, USA.
Seema AgarwalDepartment of Pathology, Center for Cell Reprogramming, Georgetown University Medical Center, Washington, DC 20007, USA.ORCID 0000-0002-8854-252X
Georgetown University · USCenter for Biologics Evaluation and Research · USYale University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Circulating tumor cells (CTCs) represent a unique population of cells that can be used to investigate the mechanistic underpinnings of metastasis. Unfortunately, current technologies designed for the isolation and capture of CTCs are inefficient. Existing literature for in vitro CTC cultures report low (6-20%) success rates. Here, we describe a new method for the isolation and culture of CTCs. Once optimized, we employed the method on 12 individual metastatic breast cancer patients and successfully established CTC cultures from all 12 samples. We demonstrate that cells propagated were of breast and epithelial origin. RNA-sequencing and pathway analysis demonstrated that CTC cultures were distinct from cells obtained from healthy donors. Finally, we observed that CTC cultures that were associated with CD45

Indexed as

Cancer metastasiscell culturecirculating tumor cellsleukocytestumor-associated neutrophils

Identifiers

PMID32998338
PMCPMC7599955
OpenAlexW3089159709

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.