ArticleLab on a chip2020
Microfluidics-enabled 96-well perfusion system for high-throughput tissue engineering and long-term all-optical electrophysiology.
Article in Lab on a chip, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Response of human iPSC-cardiomyocytes to adrenergic drugs assessed by high-throughput pericellular oxygen measurements and computational modeling.Journal of molecular and cellular cardiology plus · 2026Article
- Augmented 3D Printing for Multiscale Microphysiological Systems.Small (Weinheim an der Bergstrasse, Germany) · 2025Review
- Pericellular oxygen dynamics in human cardiac fibroblasts and iPSC-cardiomyocytes in high-throughput plates: insights from experiments and modeling.Journal of molecular and cellular cardiology plus · 2025Article
- Synergistic potential of stem cells and microfluidics in regenerative medicine.Molecular and cellular biochemistry · 2025Review
- Microfluidic platforms for monitoring cardiomyocyte electromechanical activity.Microsystems & nanoengineering · 2025Review
- Novel, low-cost bioreactor forFrontiers in bioengineering and biotechnology · 2025Article
- Force versus Response: Methods for Activating and Characterizing Mechanosensitive Ion Channels and GPCRs.Advanced healthcare materials · 2024Review
- A miniaturized culture platform for control of the metabolic environment.Biomicrofluidics · 2024Article
- Multi-well plate lid for single-step pooling of 96 samples for high-throughput barcode-based sequencing.Biomedical microdevices · 2024Article
- A standard 96-well based high throughput microfluidic perfusion biofilm reactor for in situ optical analysis.Biomedical microdevices · 2023Article
- High-throughput optical sensing of peri-cellular oxygen in cardiac cells: system characterization, calibration, and testing.bioRxiv : the preprint server for biology · 2023Article
- A 96-wells fluidic system for high-throughput screenings under laminar high wall shear stress conditions.Microsystems & nanoengineering · 2023Article
- High-throughput optical sensing of peri-cellular oxygen in cardiac cells: system characterization, calibration, and testing.Frontiers in bioengineering and biotechnology · 2023Article
- Will microfluidics enable functionally integrated biohybrid robots?Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- MatriGridBioengineering (Basel, Switzerland) · 2022Review
- Engineering complexity in human tissue models of cancer.Advanced drug delivery reviews · 2022Review
- Human iPSC-Cardiomyocytes as an Experimental Model to Study Epigenetic Modifiers of Electrophysiology.Cells · 2022Review
- Sample Preparation and Diagnostic Methods for a Variety of Settings: A Comprehensive Review.Molecules (Basel, Switzerland) · 2021Review
- Fabrication approaches for high-throughput and biomimetic disease modeling.Acta biomaterialia · 2021Review
- Spheroid-on-chip microfluidic technology for the evaluation of the impact of continuous flow on metastatic potential in cancer modelsBiomicrofluidics · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
This work demonstrates a novel high-throughput (HT) microfluidics-enabled uninterrupted perfusion system (HT-μUPS) and validates its use with chronic all-optical electrophysiology in human excitable cells. HT-μUPS consists of a soft multichannel microfluidic plate cover which could button on a commercial HT 96-well plate. Herein, we demonstrate the manufacturing process of the system and its usages in acute and chronic all-optical electrophysiological studies of human induced pluripotent stem-cell-derived cardiomyocytes (iPSC-CM) and engineered excitable (spiking HEK) cells. HT-μUPS perfusion maintained functional voltage and calcium responses in iPSC-CM and spiking HEK cells under spontaneous conditions and under optogenetic pacing. Long-term culture with HT-μUPS improved cell viability and optogenetically-tracked calcium responses in spiking HEK cells. The simplicity of this design and its compatibility with HT all-optical electrophysiology can empower cell-based assays for personalized medicine using patient-derived cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.