ArticleAdvanced biosystems2020
Cryopreservation and Laser Nanowarming of Zebrafish Embryos Followed by Hatching and Spawning.
Article in Advanced biosystems, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- TiACS omega · 2026Article
- Recent Advances in Cryopreservation of Animal Genetic Resources: Principles, Influencing Factors, and Material-Specific Challenges.Animals : an open access journal from MDPI · 2026Review
- Nanotechnology in reproductive medicine: from gamete engineering to precision therapeutics.RSC advances · 2026Review
- Molecular Understanding of Cryopreservation of Marine Invertebrates: Achievements and New Perspectives.Cell biochemistry and biophysics · 2026Review
- Cryopreservation toxicity and morphological outcomes inAnimal reproduction · 2026Article
- Advanced Rewarming Technologies for Cryopreservation.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Chilling injury mechanism in the immature oocytes of zebrafish (Danio rerio).The Journal of reproduction and development · 2025Article
- The mechanism of heat stress-induced injury in the immature oocytes of zebrafish (Danio rerio).The Journal of reproduction and development · 2025Article
- Cryopreservation of juvenile Mytilus galloprovincialis to safeguard mollusk biodiversity and support aquaculture.Scientific reports · 2025Article
- The Sixth Mass Extinction and Amphibian Species Sustainability Through Reproduction and Advanced Biotechnologies, Biobanking of Germplasm and Somatic Cells, and Conservation Breeding Programs (RBCs).Animals : an open access journal from MDPI · 2024Review
- 3D Printed Organisms Enabled by Aspiration-Assisted Adaptive Strategies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- De novo design of a nanoregulator for the dynamic restoration of ovarian tissue in cryopreservation and transplantation.Journal of nanobiotechnology · 2024Review
- High-throughput genetic manipulation of multicellular organisms using a machine-vision guided embryonic microinjection robot.Genetics · 2024Article
- The Journal of law, medicine & ethics : a journal of the American Society of Law, Medicine & Ethics · 2024Article
- Conduction-Dominated Cryomesh for Organism Vitrification.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Rethinking the application of nanoparticles in women's reproductive health and assisted reproduction.Nanomedicine (London, England) · 2024Review
- Zebrafish as a high throughput model for organ preservation and transplantation research.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- Cryopreservation and revival of Hawaiian stony corals using isochoric vitrification.Nature communications · 2023Article
- Incorporate delivery, warming and washing methods into efficient cryopreservation.Frontiers in bioengineering and biotechnology · 2023Article
- Rapid joule heating improves vitrification based cryopreservation.Nature communications · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
This study shows for the first time the ability to rewarm cryopreserved zebrafish embryos that grow into adult fish capable of breeding normally. The protocol employs a single injection of cryoprotective agents (CPAs) and gold nanorods (GNRs) into the yolk and immersion in a precooling bath to dehydrate the perivitelline space. Then embryos are encapsulated within CPA and GNR droplets, plunged into liquid nitrogen, cryogenically stabilized, and rewarmed by a laser pulse. Postlaser nanowarming, embryos (n = 282) exhibit intact structure by 1 h (40%), continued development after 3 h (22%), movement after 24 h (11%), hatching after 48 h (9%), and swimming after Day 5 (3%). Finally, from fish that survives till Day 5, two larvae are grown to adulthood and spawned, yielding survival comparable to an unfrozen control. Future efforts will focus on improving the survival to adulthood and developing methods to cryopreserve large numbers of embryos for research, aquaculture, and biodiversity preservation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.