Evidence map›Paper›PMID 32996063›Full record

ArticleReproductive sciences (Thousand Oaks, Calif.)2021

Protective Effects of Puerarin on Premature Ovarian Failure via Regulation of Wnt/β-catenin Signaling Pathway and Oxidative Stress.

Cheng Chen, Song Li, Cong Hu, Weiwei Cao, Qingfeng Fu, Jia Li, Liping Zheng, Jian Huang

Abstract read
PubMed Publisher
In one paragraph

Article in Reproductive sciences (Thousand Oaks, Calif.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed, 2 pooled it
2.9field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 2 syntheses or guidelines pooled it, 41 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Cheng ChenJiangxi Medical College Nanchang University, Jiangxi Province, 330006, Nanchang, China.ORCID https://orcid.org/0000-0002-2495-7435
Song LiJiangxi Medical College Nanchang University, Jiangxi Province, 330006, Nanchang, China.ORCID https://orcid.org/0000-0002-1664-6290
Cong HuJiangxi Medical College Nanchang University, Jiangxi Province, 330006, Nanchang, China.ORCID https://orcid.org/0000-0001-6503-7750
Weiwei CaoJiangxi Medical College Nanchang University, Jiangxi Province, 330006, Nanchang, China.ORCID https://orcid.org/0000-0002-8434-2221
Qingfeng FuJiangxi Medical College Nanchang University, Jiangxi Province, 330006, Nanchang, China.ORCID https://orcid.org/0000-0002-3170-8920
Jia LiJiangxi Medical College Nanchang University, Jiangxi Province, 330006, Nanchang, China.ORCID https://orcid.org/0000-0003-4072-9034
Liping ZhengJiangxi Medical College Nanchang University, Jiangxi Province, 330006, Nanchang, China.ORCID https://orcid.org/0000-0001-6102-2332
Jian HuangJiangxi Medical College Nanchang University, Jiangxi Province, 330006, Nanchang, China. 598490492@qq.com.ORCID https://orcid.org/0000-0002-7401-4662
Nanchang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study was designed to investigate the protective effects of puerarin (PUE), which work via the Wnt/β-catenin signaling pathway, and oxidative stress in the premature ovarian failure (POF) model. Two-month-old female mice were randomly divided into four groups. One group was used as the control, and the other three groups were injected with cyclophosphamide and busulfan to create POF models. Two POF treatment groups were gavaged with 100 or 200 mg/kg PUE for 28 days. Next, the ovaries were fixed, and the numbers of different stage follicles were measured, and the ovarian surface epithelium (OSE) was collected. Oct4 and Mvh expression, Wnt/β-catenin signaling pathway activity, the oxidative stress factors SOD2 and Nrf2, and the apoptosis-related proteins Bcl-2 and Bax were detected by IHC, RT-QPCR, and western blotting. We found that the number of follicles, Oct4 and Mvh expression, and Wnt/β-catenin-signaling activity were reduced in the POF groups (p < 0.05 or p < 0.001). After PUE treatment, the follicle number and the primordial follicle ratio increased (p < 0.01), while the atresia ratio decreased (p < 0.01). In addition, the expression levels of Oct4, Mvh, Wnt1, β-catenin, cyclin D1, SOD2, and Nrf2 showed obvious recovery compared with levels in the POF group (p < 0.01, p < 0.05, or p < 0.001). The Bcl-2/Bax ratio in the POF model had reduced by about 60% compared with the control group (p < 0.001) and improved by about 50% after PUE treatment (p < 0.001). In conclusion, PUE may improve the survival of female reproductive stem cells (FGSCs) and play a protective role against POF via a mechanism involving the Wnt/β-catenin signaling pathway, as well as relieving oxidative stress. Further investigations should focus on the culture of oocytes and FGSCs in vitro in a PUE environment with inhibitors or agonists of the Wnt signaling pathway.

Indexed as

AnimalsApoptosisBusulfanCyclophosphamideDisease Models, AnimalFemaleIsoflavonesMiceOvarian FollicleOxidative StressPrimary Ovarian InsufficiencyProtective AgentsWnt Signaling PathwayBusulfanCyclophosphamideIsoflavonesProtective AgentspuerarinFemale reproductive stem cellsOxidative stressPremature ovarian failurePuerarinWnt/β-catenin pathway

Identifiers

PMID32996063
OpenAlexW3091098776

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.