Evidence map›Paper›PMID 32996049›Full record

ArticleParasitology research2020

Longitudinal detection of Cryptosporidium spp. in 1-10-week-old dairy calves on a farm in Xinjiang, China.

Meng Qi, Kuankuan Zhang, Meigui Huang, Siqi Wang, Chunyan Xu, Tian Wang, Bo Jing, Junqiang Li

Abstract read
PubMed Publisher
In one paragraph

Article in Parasitology research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 22 citations in OpenAlex.

  1. Molecular Prevalence andAnimals : an open access journal from MDPI · 2026
    Article
  2. Article
  3. Article
  4. Current research in parasitology & vector-borne diseases · 2025
    Review
  5. Article
  6. Molecular characterization and zoonotic risk assessment ofOne health (Amsterdam, Netherlands) · 2024
    Article
  7. Article
  8. Article
  9. Article
  10. First report ofFrontiers in veterinary science · 2023
    Article
  11. Review
  12. Development and Application of aMicroorganisms · 2021
    Article
  13. Review
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Meng QiCollege of Animal Science, Tarim University, Tarim Road 1487, Alar, 843300, Xinjiang, People's Republic of China.
Kuankuan ZhangCollege of Animal Science, Tarim University, Tarim Road 1487, Alar, 843300, Xinjiang, People's Republic of China.
Meigui HuangCollege of Animal Science, Tarim University, Tarim Road 1487, Alar, 843300, Xinjiang, People's Republic of China.
Siqi WangCollege of Animal Science, Tarim University, Tarim Road 1487, Alar, 843300, Xinjiang, People's Republic of China.
Chunyan XuCollege of Animal Science, Tarim University, Tarim Road 1487, Alar, 843300, Xinjiang, People's Republic of China.
Tian WangCollege of Animal Science, Tarim University, Tarim Road 1487, Alar, 843300, Xinjiang, People's Republic of China.
Bo JingCollege of Animal Science, Tarim University, Tarim Road 1487, Alar, 843300, Xinjiang, People's Republic of China. jingbodky@126.com.
Junqiang LiAcademy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou, 450046, China. lijunqiangcool@126.com.ORCID http://orcid.org/0000-0002-6417-0901
Tarim University · CNFirst Affiliated Hospital of Henan University · CN

Funding

National Natural Science Foundation of China 31702227Program for Young and Middle-aged Leading Science, Technology, and Innovation of Xinjiang Production & Construction Group 2018CB034
6 · The paper itself

Abstract

Cryptosporidiosis is an important cause of morbidity and mortality in the cattle industry and leads to severe economic losses. Fecal samples were collected from 25 dairy calves once a week for 10 weeks for continuous longitudinal detection of Cryptosporidium spp. Cryptosporidium spp. were detected via nested PCR amplification of the ribosomal small subunit RNA gene, followed by restriction fragment length polymorphism analysis with enzymes SspI and MboII to identify the species. PCR results indicated that all calves were infected with Cryptosporidium spp. at least once, with an average overall prevalence rate of 52.0% (130/250). One-week-old calves had the highest occurrences of Cryptosporidium infection (96.0%), 2-week-old calves (80.0%) had the second highest, and calves with watery diarrhea also had a higher occurrence of infection (92.3%). Four Cryptosporidium species, C. parvum, C. bovis, C. ryanae, and C. andersoni, were identified, with C. parvum being the most common. Forty-eight C. parvum isolates were further subtyped via nested PCR amplification of the 60-kDa glycoprotein gene, and all were identified as subtype IIdA15G1. The results demonstrated that C. parvum mainly infects dairy calves which are younger than 3 weeks old.

Indexed as

Cattle DiseasesCryptosporidiosisAnimalsCattleChinaCryptosporidiumFarmsFecesGenes, rRNALongitudinal StudiesPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthPrevalenceCryptosporidiosisDairy calvesLongitudinal detectionXinjiang

Identifiers

PMID32996049
OpenAlexW3089740396

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.