ArticleSynthetic biology (Oxford, England)2020
DNA-BOT: a low-cost, automated DNA assembly platform for synthetic biology.
Article in Synthetic biology (Oxford, England), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 92 citations in OpenAlex.
- Advances in large DNA fragment assembly for microbial cell factory engineering.Quantitative biology (Beijing, China) · 2026Review
- Automated linear DNA assembly of A. thaliana's chloroplast and mitochondrial genome.Nucleic acids research · 2026Article
- Slowpoke: An Automated Golden Gate Cloning Workflow for Opentrons OT-2 and Flex.ACS synthetic biology · 2026Article
- Modification of a Low-Cost Pipetting Robot for Nanoliter Liquid Handling and Autosampling for Liquid Chromatography-Mass Spectrometry.Journal of separation science · 2026Article
- Automated Genetic Manipulation for the Construction ofChem & bio engineering · 2025Article
- ActinoMation: A literate programming approach for medium-throughput robotic conjugation ofSynthetic and systems biotechnology · 2025Article
- Semi-automated workflow for high-throughput Agrobacterium-mediated plant transformation.The Plant journal : for cell and molecular biology · 2025Article
- Fine-Tuning Genetic Circuits via Host Context and RBS Modulation.ACS synthetic biology · 2025Article
- OT-Mation: an open-source code for parsing CSV files into Python scripts for control of OT-2 liquid-handling robotics.Synthetic biology (Oxford, England) · 2025Article
- Optimizing Yeast Homologous Recombination for Splicing Large Coronavirus Genome Fragments.International journal of molecular sciences · 2024Article
- Engineering biology and automation-Replicability as a design principle.Engineering biology · 2024Review
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- Automated high-throughput DNA synthesis and assembly.Heliyon · 2024Review
- Technical assessment of different extraction methods and transcriptome profiling of RNA isolated from small volumes of blood.Scientific reports · 2023Article
- Technical upgrade of an open-source liquid handler to support bacterial colony screening.Frontiers in bioengineering and biotechnology · 2023Article
- AssemblyTron: flexible automation of DNA assembly with Opentrons OT-2 lab robots.Synthetic biology (Oxford, England) · 2023Article
- The automated Galaxy-SynBioCAD pipeline for synthetic biology design and engineering.Nature communications · 2022Article
- Standardization of Synthetic Biology Tools and Assembly Methods forACS synthetic biology · 2022Review
- LOICA: Integrating Models with Data for Genetic Network Design Automation.ACS synthetic biology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multi-part DNA assembly is the physical starting point for many projects in Synthetic and Molecular Biology. The ability to explore a genetic design space by building extensive libraries of DNA constructs is essential for creating programmed biological systems. With multiple DNA assembly methods and standards adopted in the Synthetic Biology community, automation of the DNA assembly process is now receiving serious attention. Automation will enable larger builds using less researcher time, while increasing the accessible design space. However, these benefits currently incur high costs for both equipment and consumables. Here, we address this limitation by introducing low-cost DNA assembly with BASIC on OpenTrons (DNA-BOT). For this purpose, we developed an open-source software package and demonstrated the performance of DNA-BOT by simultaneously assembling 88 constructs composed of 10 genetic parts, evaluating the promoter, ribosome binding site and gene order design space for a three-gene operon. All 88 constructs were assembled with high accuracy, at a consumables cost of $1.50-$5.50 per construct. This illustrates the efficiency, accuracy and affordability of DNA-BOT, making it accessible for most labs and democratizing automated DNA assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.