ArticleSynthetic biology (Oxford, England)2018
Cell-free prototyping strategies for enhancing the sustainable production of polyhydroxyalkanoates bioplastics.
Article in Synthetic biology (Oxford, England), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The trial behind it
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Who cites it
19 citing papers in PubMed, 58 citations in OpenAlex.
- Enzymatic and microbial routes to bioplastics: The green chemistry frontier of biopolymers.FEBS open bio · 2026Review
- Design-driven optimization of low-cost reagent formulations for reproducible and high-yielding cell-free gene expression.Nature communications · 2026Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Carbon Negative Synthesis of Amino Acids Using a Cell-Free-Based Biocatalyst.ACS synthetic biology · 2024Article
- Cell-Free Synthesis: Expediting Biomanufacturing of Chemical and Biological Molecules.Molecules (Basel, Switzerland) · 2024Review
- Article
- Cell-Free Expression of a Therapeutic Protein Serratiopeptidase.Molecules (Basel, Switzerland) · 2023Article
- Cell-free expression of NO synthase and P450 enzyme for the biosynthesis of an unnatural amino acid L-4-nitrotryptophan.Synthetic and systems biotechnology · 2022Article
- Variability in cell-free expression reactions can impact qualitative genetic circuit characterization.Synthetic biology (Oxford, England) · 2022Article
- Biofoundry-assisted expression and characterization of plant proteins.Synthetic biology (Oxford, England) · 2021Article
- Designing Modular Cell-free Systems for Tunable Biotransformation of l-phenylalanine to Aromatic Compounds.Frontiers in bioengineering and biotechnology · 2021Article
- Aldehyde Production in Crude Lysate- and Whole Cell-Based Biotransformation Using a Noncanonical Redox Cofactor System.ACS catalysis · 2020Article
- In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design.Nature chemical biology · 2020Article
- Metabolic engineering of Escherichia coli for optimized biosynthesis of nicotinamide mononucleotide, a noncanonical redox cofactor.Microbial cell factories · 2020Article
- Multicomponent Microscale Biosynthesis of Unnatural Cyanobacterial Indole Alkaloids.ACS synthetic biology · 2020Article
- Cell-free gene expression: an expanded repertoire of applications.Nature reviews. Genetics · 2020Review
- Biological Materials: The Next Frontier for Cell-Free Synthetic Biology.Frontiers in bioengineering and biotechnology · 2020Review
- Streamlining the preparation of "endotoxin-free" ClearColi cell extract with autoinduction media for cell-free protein synthesis of the therapeutic protein crisantaspase.Synthetic and systems biotechnology · 2019Article
- Cell-free biosynthesis of limonene using enzyme-enrichedSynthetic biology (Oxford, England) · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The polyhydroxyalkanoates (PHAs) are microbially-produced biopolymers that could potentially be used as sustainable alternatives to oil-derived plastics. However, PHAs are currently more expensive to produce than oil-derived plastics. Therefore, more efficient production processes would be desirable. Cell-free metabolic engineering strategies have already been used to optimize several biosynthetic pathways and we envisioned that cell-free strategies could be used for optimizing PHAs biosynthetic pathways. To this end, we developed several
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.