ReviewSynthetic biology (Oxford, England)2018
Extrinsic stochastic factors (solute partition) in gene expression inside lipid vesicles and lipid-stabilized water-in-oil droplets: a review.
Review in Synthetic biology (Oxford, England), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
- Hybrid Protocells Based on Coacervate-Templated Fatty Acid Vesicles Combine Improved Membrane Stability with Functional Interior Protocytoplasm.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- The interaction of a self-assembled nanoparticle and a lipid membrane: Binding, disassembly and re-distribution.Heliyon · 2024Article
- Chemical Systems for Wetware Artificial Life: Selected Perspectives in Synthetic Cell Research.International journal of molecular sciences · 2023Article
- Membranous and Membraneless Interfaces-Origins of Artificial Cellular Complexity.Life (Basel, Switzerland) · 2023Article
- Measuring Encapsulation Efficiency in Cell-Mimicking Giant Unilamellar Vesicles.ACS synthetic biology · 2023Article
- Light energy transduction in liposome-based artificial cells.Frontiers in bioengineering and biotechnology · 2023Review
- Chromatophores efficiently promote light-driven ATP synthesis and DNA transcription inside hybrid multicompartment artificial cells.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A MATLAB toolbox for modeling genetic circuits in cell-free systems.Synthetic biology (Oxford, England) · 2021Article
- Programmed spatial organization of biomacromolecules into discrete, coacervate-based protocells.Nature communications · 2020Article
- Artificial cells drive neural differentiation.Science advances · 2020Article
- Intercellular communication between artificial cells by allosteric amplification of a molecular signal.Nature communications · 2020Article
- Cell-Free Systems: A Proving Ground for Rational Biodesign.Frontiers in bioengineering and biotechnology · 2020Article
- Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology.Frontiers in bioengineering and biotechnology · 2020Review
- Is Research on "Synthetic Cells" Moving to the Next Level?Life (Basel, Switzerland) · 2018Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The encapsulation of transcription-translation (TX-TL) machinery inside lipid vesicles and water-in-oil droplets leads to the construction of cytomimetic systems (often called 'synthetic cells') for synthetic biology and origins-of-life research. A number of recent reports have shown that protein synthesis inside these microcompartments is highly diverse in terms of rate and amount of synthesized protein. Here, we discuss the role of extrinsic stochastic effects (i.e. solute partition phenomena) as relevant factors contributing to this pattern. We evidence and discuss cases where between-compartment diversity seems to exceed the expected theoretical values. The need of accurate determination of solute content inside individual vesicles or droplets is emphasized, aiming at validating or rejecting the predictions calculated from the standard fluctuations theory. At the same time, we promote the integration of experiments and stochastic modeling to reveal the details of solute encapsulation and intra-compartment reactions.
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