ArticleIranian journal of microbiology2020
Aptamer-nanobody based ELASA for detection of
Article in Iranian journal of microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 16 citations in OpenAlex.
- A novel DNA aptamer for Vibrio cholerae O139 detection: a whole-cell SELEX approach.Molecular biology reports · 2025Article
- A review on camelid nanobodies with potential application in veterinary medicine.Veterinary research communications · 2024Review
- A Label-Free Colorimetric AuNP-Aptasensor for the Rapid Detection ofCellular and molecular bioengineering · 2024Article
- Review of Rapid Diagnostic Methods forThe East African health research journal · 2023Article
- A Novel Nanobody-Horseradish Peroxidase Fusion Based-Competitive ELISA to Rapidly Detect Avian Corona-Virus-Infectious Bronchitis Virus Antibody in Chicken Serum.International journal of molecular sciences · 2022Article
- Development of nanobody-horseradish peroxidase-based sandwich ELISA to detect Salmonella Enteritidis in milk and in vivo colonization in chicken.Journal of nanobiotechnology · 2022Article
- Aptamers and Aptamer-Coupled Biosensors to Detect Water-Borne Pathogens.Frontiers in microbiology · 2021Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectivesIn recent years, the prevalence of diseases caused by MATERIALS AND
methodsIn this research high-affinity DNA aptamers against with two major serotypes of Inaba (ATCC 39315) and Ogawa (clinical sample) were selected from DNA aptamer library through 12 rounds of Systematic Evolution of Ligands by Exponential (SELEX) enrichment procedure using live cells as a target which monitored with flow cytometry.
resultsThe binding efficiency and dissociation constant of the isolated aptamers V.ch47 and V.ch27 were 56.4%, 53.3% and 15.404 ± 4.776 pM, 20.186 ± 3.655 pM, respectively. A sandwich Enzyme-linked aptamer sorbent assay (ELASA) was developed with the biotinylated V.ch47 aptamer and our previously developed nanobody anti-Lipopolysaccharides (LPS). We optimized this system with
conclusionOur results showed that the sandwich ELASA is sensitive enough for the rapid detection of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.