ArticleScientific reports2020
The use of high resolution melting analysis of ITS-1 for rapid differentiation of parasitic nematodes Haemonchus contortus and Ashworthius sidemi.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 18 citations in OpenAlex.
- Hunting for Helminths: short- and long-read shotgun metagenomics for helminth detection in faecal samples.PLoS neglected tropical diseases · 2026Article
- Phylogenetic and molecular insights into the genetic diversity of the invasive nematodeInternational journal for parasitology. Parasites and wildlife · 2026Article
- Sharing more than just the pastures? Parasitological monitoring of translocated European bison (International journal for parasitology. Parasites and wildlife · 2026Article
- Molecular uncovering of important helminth species in wild ruminants in the Czech Republic.Frontiers in veterinary science · 2025Article
- A molecular assessment of Ostertagia leptospicularis and Spiculopteragia asymmetrica among wild fallow deer in Northern Ireland and implications for false detection of livestock-associated species.Parasites & vectors · 2024Article
- Low-cost molecular methods to characterise gastrointestinal nematode co-infections of goats in Africa.Parasites & vectors · 2023Article
- Soil-Borne Nematodes: Impact in Agriculture and Livestock and Sustainable Strategies of Prevention and Control with Special Reference to the Use of Nematode Natural Enemies.Pathogens (Basel, Switzerland) · 2022Review
- Helminth fauna of the Eurasian beaver in the Czech Republic with remarks on the genetic diversity of specialist Stichorchis subtriquetrus (Digenea: Cladorchiidae).Parasitology research · 2022Article
- Article
- The identification and semi-quantitative assessment of gastrointestinal nematodes in faecal samples using multiplex real-time PCR assays.Parasites & vectors · 2021Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Among gastrointestinal nematodes, haematophagous strongylids Haemonchus contortus and Ashworthius sidemi belong to the most pathogenic parasites of both domestic and wild ruminants. Correct identification of parasitic taxa is of crucial importance in many areas of parasite research, including monitoring of occurrence, epidemiological studies, or testing of effectiveness of therapy. In this study, we identified H. contortus and A. sidemi in a broad range of ruminant hosts that occur in the Czech Republic using morphological/morphometric and molecular approaches. As an advanced molecular method, we employed qPCR followed by High Resolution Melting analysis, specifically targeting the internal transcribed spacer 1 (ITS-1) sequence to distinguish the two nematode species. We demonstrate that High Resolution Melting curves allow for taxonomic affiliation, making it a convenient, rapid, and reliable identification tool.
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