ArticleScientific reports2020
An efficient microinjection method to generate human anaplasmosis agent Anaplasma phagocytophilum-infected ticks.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 14 citations in OpenAlex.
- Arthropod exosomal glycine-rich protein as a potential vaccine candidate effectively reduces tick blood-feeding and pathogen transmission.The EMBO journal · 2026Article
- McFarland Standards-Based Spectrophotometry Method for Calculating Approximate Multiplicity of Infection for an Obligate Intracellular BacteriumMicroorganisms · 2025Article
- Arthropod autophagy molecules facilitate Anaplasma phagocytophilum infection of Ixodes scapularis tick cells.Communications biology · 2025Article
- Antibody-blocking of a tick transporter impairs Anaplasma phagocytophilum colonization in Haemaphysalis longicornis ticks.Scientific reports · 2024Article
- Long-lasting infection with Anaplasma ovis in sheep.Veterinary research communications · 2024Article
- The anal pore route is efficient to infectFrontiers in cellular and infection microbiology · 2023Article
- Seroprevalence canine survey for selected vector-borne pathogens and its relationship with poverty in metropolitan Pereira, Colombia, 2020.Parasite epidemiology and control · 2022Article
- Rickettsial Pathogen Perturbs Tick Circadian Gene to Infect the Vertebrate Host.International journal of molecular sciences · 2022Article
- Direct injection of Amblyomma americanum ticks with Cytauxzoon felis.Ticks and tick-borne diseases · 2022Article
- An Experimental Murine Model to Study Acquisition Dynamics of Tick-Borne Langat Virus inFrontiers in microbiology · 2022Article
- Molecular Detection of Zoonotic and Non-Zoonotic Pathogens from Wild Boars and Their Ticks in the Corsican Wetlands.Pathogens (Basel, Switzerland) · 2021Article
- Double anus in an Ixodes scapularis nymph, a medically important tick vector.Parasites & vectors · 2021Article
- Update on prevalence and distribution pattern of tick-borne diseases among humans in India: a review.Parasitology research · 2021Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Ticks are important vectors that transmit several pathogens including human anaplasmosis agent, Anaplasma phagocytophilum. This bacterium is an obligate intracellular rickettsial pathogen. An infected reservoir animal host is often required for maintenance of this bacterial colony and as a source for blood to perform needle inoculations in naïve animals for tick feeding studies. In this study, we report an efficient microinjection method to generate A. phagocytophilum-infected ticks in laboratory conditions. The dense-core (DC) form of A. phagocytophilum was isolated from in vitro cultures and injected into the anal pore of unfed uninfected Ixodes scapularis nymphal ticks. These ticks successfully transmitted A. phagocytophilum to the murine host. The bacterial loads were detected in murine blood, spleen, and liver tissues. In addition, larval ticks successfully acquired A. phagocytophilum from mice that were previously infected by feeding with DC-microinjected nymphal ticks. Transstadial transmission of A. phagocytophilum from larvae to nymphal stage was also evident in these ticks. Taken together, our study provides a timely, rapid, and an efficient method not only to generate A. phagocytophilum-infected ticks but also provides a tool to understand acquisition and transmission dynamics of this bacterium and perhaps other rickettsial pathogens from medically important vectors.
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